Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, mouse liver tissue, L02 cells |
| Positive IHC detected in | mouse liver tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 3 publications below |
Product Information
20784-1-AP targets ANO6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ANO6 fusion protein Ag13937 相同性解析による交差性が予測される生物種 |
| Full Name | anoctamin 6 |
| Calculated molecular weight | 910 aa, 106 kDa |
| Observed molecular weight | 95 kDa |
| GenBank accession number | BC098410 |
| Gene Symbol | ANO6 |
| Gene ID (NCBI) | 196527 |
| RRID | AB_2878737 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q4KMQ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ANO6 antibody 20784-1-AP | Download protocol |
| IHC protocol for ANO6 antibody 20784-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Medicine (Baltimore) ANO6 is a reliable prognostic biomarker and correlates to macrophage polarization in breast cancer | ||
Lab Med Identification of biomarkers associated with pathological tumor staging and their utility in the diagnosis and prognosis of prostate cancer | ||
Metabolites Decreased Hyocholic Acid and Lysophosphatidylcholine Induce Elevated Blood Glucose in a Transgenic Porcine Model of Metabolic Disease | ||
Open Med (Wars) ANO6 (TMEM16F) inhibits gastrointestinal stromal tumor growth and induces ferroptosis | ||