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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANP32A Monoclonal antibody (67687-1-Ig)

    WB analysis using 67687-1-Ig (same clone as 67687-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67687-1-Ig (ANP32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ANP32A Monoclonal antibody (67687-1-Ig)

    WB analysis using 67687-1-Ig (same clone as 67687-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67687-1-Ig (ANP32A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ANP32A Monoclonal antibody (67687-1-Ig)

    IHC staining of mouse brain using 67687-1-Ig (same clone as 67687-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ANP32A Monoclonal antibody (67687-1-Ig)

    IHC staining of mouse brain using 67687-1-Ig (same clone as 67687-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ANP32A Monoclonal antibody (67687-1-Ig)

    IHC staining of human kidney using 67687-1-Ig (same clone as 67687-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ANP32A Monoclonal antibody (67687-1-Ig)

    IHC staining of human kidney using 67687-1-Ig (same clone as 67687-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67687-1-Ig (ANP32A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ANP32A Monoclonal antibody (67687-1-Ig)

    IF Staining of A431 using 67687-1-Ig (same clone as 67687-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32A antibody (67687-1-Ig, Clone: 2D8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ANP32A Monoclonal antibody (67687-1-Ig)

    IF Staining of A431 using 67687-1-Ig (same clone as 67687-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANP32A antibody (67687-1-Ig, Clone: 2D8C5 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67687-1-PBS in a different storage buffer formulation.

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ANP32A Monoclonal antibody, PBS Only

ANP32A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67687-1-PBS
Clone No.2D8C5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Leucine-rich acidic nuclear protein, LANP, I1PP2A, C15orf1, Acidic leucine-rich nuclear phosphoprotein 32 family member A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67687-1-PBS targets ANP32A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ANP32A fusion protein Ag8546 相同性解析による交差性が予測される生物種
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
Calculated molecular weight 249 aa, 29 kDa
Observed molecular weight29 kDa
GenBank accession numberBC007200
Gene Symbol ANP32A
Gene ID (NCBI) 8125
RRIDAB_2882880
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP39687
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.