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KD/KO Validated

ANP32B Polyclonal antibody

Name: ANP32B Polyclonal antibody
Brand: Proteintech
SKU: 10843-1-AP

ANP32B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10843-1-AP

Synonyms

Silver-stainable protein SSP29, Putative HLA-DR-associated protein I-2, PHAPI2, APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B

Validation Data Gallery

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AllWBIHCIF/ICC

WB analysis of HEK-293 using 10843-1-AP
WB result of ANP32B antibody (10843-1-AP, 1:5000) with si-control and si-ANP32B transfected HEK293 cells.
WB analysis using 10843-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10843-1-AP (ANP32B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of RAW 264.7 using 10843-1-AP
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10843-1-AP (ANP32B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10843-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10843-1-AP (ANP32B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10843-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10843-1-AP (ANP32B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 10843-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10843-1-AP (ANP32B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 10843-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10843-1-AP (ANP32B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 10843-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10843-1-AP (ANP32B Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human pancreas using 10843-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10843-1-AP (ANP32B Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human colon cancer using 10843-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10843-1-AP (ANP32B antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HeLa using 10843-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ANP32B antibody (10843-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Cat No. 10843-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, HEK-293 cells, HeLa cells, HEK-293T cells
Positive IHC detected in	human colon tissue, human colon cancer tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 10 publications below
IHC	See 1 publications below

Product Information

10843-1-AP targets ANP32B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ANP32B fusion protein Ag1274 相同性解析による交差性が予測される生物種
Full Name	acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
Calculated molecular weight	29 kDa
Observed molecular weight	29 kDa
GenBank accession number	BC013003
Gene symbol	ANP32B
Gene ID (NCBI)	10541
RRID	AB_2056460
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).

Protocols

Product Specific Protocols
WB protocol for ANP32B antibody 10843-1-AP	Download protocol
IHC protocol for ANP32B antibody 10843-1-AP	Download protocol
IF protocol for ANP32B antibody 10843-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Sci Adv The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals Authors - Liuke Sun View Article
mouse	WB	Proc Natl Acad Sci U S A Acidic nuclear phosphoprotein 32kDa (ANP32)B-deficient mouse reveals a hierarchy of ANP32 importance in mammalian development. Authors - Reilly Patrick T PT View Article
human	WB	Carcinogenesis Downregulation of ANP32B, a novel substrate of caspase-3, enhances caspase-3 activation and apoptosis induction in myeloid leukemic cells. Authors - Shen Shao-Ming SM KD Validated View Article
human	WB	Blood Cancer J PMID: 24413065 Authors - B C Valdez View Article
human	WB,IHC	Cell Death Dis ANP32B deficiency impairs proliferation and suppresses tumor progression by regulating AKT phosphorylation. Authors - S Yang KD Validated View Article
human	WB	J Virol ANP32 proteins are essential for influenza virus replication in human cells. Authors - Ecco Staller KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANP32B Polyclonal antibody

ANP32B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 10843-1-AP

WB analysis using 10843-1-AP

WB analysis of RAW 264.7 using 10843-1-AP

WB analysis of A431 using 10843-1-AP

WB analysis of HeLa using 10843-1-AP

IHC staining of human colon using 10843-1-AP

IHC staining of human colon using 10843-1-AP

IHC staining of human pancreas using 10843-1-AP

IHC staining of human pancreas using 10843-1-AP

IHC staining of human colon cancer using 10843-1-AP

IF Staining of HeLa using 10843-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals

Acidic nuclear phosphoprotein 32kDa (ANP32)B-deficient mouse reveals a hierarchy of ANP32 importance in mammalian development.

Downregulation of ANP32B, a novel substrate of caspase-3, enhances caspase-3 activation and apoptosis induction in myeloid leukemic cells.

PMID: 24413065

ANP32B deficiency impairs proliferation and suppresses tumor progression by regulating AKT phosphorylation.

ANP32 proteins are essential for influenza virus replication in human cells.