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  • Featured Product
  • KD/KO Validated

ANTXR1 Polyclonal antibody, PBS Only

ANTXR1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15091-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using ANTXR1 Polyclonal antibody (15091-1-AP)

    WB analysis of mouse testis using 15091-1-AP (same clone as 15091-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ANTXR1 Polyclonal antibody (15091-1-AP)

    WB analysis of HeLa using 15091-1-AP (same clone as 15091-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-13 cells using ANTXR1 Polyclonal antibody (15091-1-AP)

    WB analysis of PC-13 using 15091-1-AP (same clone as 15091-1-PBS)

    PC-13 cells were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using ANTXR1 Polyclonal antibody (15091-1-AP)

    WB analysis of mouse lung using 15091-1-AP (same clone as 15091-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ANTXR1 Polyclonal antibody (15091-1-AP)

    IHC staining of human colon cancer using 15091-1-AP (same clone as 15091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15091-1-AP (TEM8 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ANTXR1 Polyclonal antibody (15091-1-AP)

    IHC staining of human colon cancer using 15091-1-AP (same clone as 15091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15091-1-AP (TEM8 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ANTXR1 Polyclonal antibody (15091-1-AP)

    IF Staining of HeLa using 15091-1-AP (same clone as 15091-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15091-1-AP (TEM8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15091-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15091-1-PBS targets ANTXR1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANTXR1 fusion protein Ag4626 相同性解析による交差性が予測される生物種
Full Name anthrax toxin receptor 1
Calculated molecular weight 63 kDa
Observed molecular weight 85 kDa, 80 kDa
GenBank accession numberBC012074
Gene symbol ANTXR1
Gene ID (NCBI) 84168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tumor endothelial marker 8 (TEM8, ANTXR1) and capillary morphogenesis protein 2 (CMG2, ANTXR2) are the two well-characterized anthrax toxin receptors, each containing a von Willebrand factor A (vWA) domain responsible for anthrax protective antigen (PA) binding (PMID: 23271637). TEM8 is a highly-conserved single-pass cell-surface protein overexpressed on tumor-infiltrating vasculature, and is thought to play a positive role in endothelial cell activities related to angiogenesis (PMID: 11559528; 15777794 ). The long isoforms of TEM8 have been identified as an 80-85 kDa doublet, and experimental deglycosylation reduced the molecular mass to 70 kDa, indicating that TEM8 undergoes multiple glycosylations (PMID: 14871805; 15689409).