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  • Featured Product
  • KD/KO Validated

Annexin A1 Polyclonal antibody

Annexin A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 21990-1-AP

Synonyms

ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Annexin A1 Polyclonal antibody (21990-1-AP)

    WB analysis using 21990-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Annexin A1 Polyclonal antibody (21990-1-AP)

    WB analysis of HeLa using 21990-1-AP

    WB result of Annexin A1 antibody (21990-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A1 transfected HeLa cells.

  • Western Blot (WB) analysis of multi-cells using Annexin A1 Polyclonal antibody (21990-1-AP)

    WB analysis of multi-cells using 21990-1-AP

    WB result of 21990-1-AP (Annexin A1 antibody).

  • Western Blot (WB) analysis of U-937 cells using Annexin A1 Polyclonal antibody (21990-1-AP)

    WB analysis of U-937 using 21990-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using Annexin A1 Polyclonal antibody (21990-1-AP)

    WB analysis of A431 using 21990-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Annexin A1 Polyclonal antibody (21990-1-AP)

    WB analysis of HepG2 using 21990-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Annexin A1 Polyclonal antibody (21990-1-AP)

    WB analysis of MCF-7 using 21990-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using Annexin A1 Polyclonal antibody (21990-1-AP)

    WB analysis of MDA-MB-453s using 21990-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using Annexin A1 Polyclonal antibody (21990-1-AP)

    WB analysis of C6 using 21990-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Annexin A1 Polyclonal antibody (21990-1-AP)

    IP experiment of HeLa using 21990-1-AP

    IP result of anti-Annexin A1 (IP:21990-1-AP, 4ug; Detection:21990-1-AP 1:20000) with HeLa cells lysate 1120 ug.

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using Annexin A1 Polyclonal antibody (21990-1-AP)

    IHC staining of human oesophagus using 21990-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using Annexin A1 Polyclonal antibody (21990-1-AP)

    IHC staining of human oesophagus using 21990-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Annexin A1 Polyclonal antibody (21990-1-AP)

    IHC staining of human ovary tumor using 21990-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Annexin A1 Polyclonal antibody (21990-1-AP)

    IHC staining of human ovary tumor using 21990-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Annexin A1 Polyclonal antibody (21990-1-AP)

    IHC staining of human kidney using 21990-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Annexin A1 Polyclonal antibody (21990-1-AP)

    IHC staining of human kidney using 21990-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Annexin A1 Polyclonal antibody (21990-1-AP)

    IHC staining of human small intestine using 21990-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Annexin A1 Polyclonal antibody (21990-1-AP)

    IHC staining of human small intestine using 21990-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using Annexin A1 Polyclonal antibody (21990-1-AP)

    IHC staining of human lung using 21990-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using Annexin A1 Polyclonal antibody (21990-1-AP)

    IHC staining of human lung using 21990-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using Annexin A1 Polyclonal antibody (21990-1-AP)

    IHC staining of human oesophagus using 21990-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using Annexin A1 Polyclonal antibody (21990-1-AP)

    IHC staining of human oesophagus using 21990-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Annexin A1 Polyclonal antibody (21990-1-AP)

    IF Staining of A549 using 21990-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Annexin A1 antibody (21990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of NIH/3T3 cells using Annexin A1 Polyclonal antibody (21990-1-AP)

    FC experiment of NIH/3T3 using 21990-1-AP

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Human Annexin A1 (21990-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA431 cells, multi-cells, MDA-MB-453s cells, HeLa cells, U-937 cells, HepG2 cells, MCF-7 cells, C6 cells, A549 cells, K-562 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman oesophagus tissue, human kidney tissue, human small intestine tissue, human ovary tumor tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21990-1-AP targets Annexin A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin A1 fusion protein Ag17273 相同性解析による交差性が予測される生物種
Full Name annexin A1
Calculated molecular weight 346 aa, 39 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC001275
Gene symbol Annexin A1
Gene ID (NCBI) 301
RRIDAB_11182596
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.

Protocols

Product Specific Protocols
WB protocol for Annexin A1 antibody 21990-1-APDownload protocol
IHC protocol for Annexin A1 antibody 21990-1-APDownload protocol
IF protocol for Annexin A1 antibody 21990-1-APDownload protocol
IP protocol for Annexin A1 antibody 21990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nature

Evolutionarily divergent mTOR remodels translatome for tissue regeneration

Authors - Olena Zhulyn
View Article
IP

Cell Host Microbe

Global mapping of Salmonella enterica-host protein-protein interactions during infection.

Authors - Philipp Walch
View Article
humanWB

Oncogene

The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis

Authors - Yapeng Wang
  • KD Validated
View Article
humanWB,IHC,IF,IP

Elife

ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein.

Authors - Horia Vais
View Article
humanWB

Mol Ther

Membrane Stabilization by Modified Steroid Offers a Potential Therapy for Muscular Dystrophy Due to Dysferlin Deficit.

Authors - Sen Chandra Sreetama
View Article
humanWB

Cancer Immunol Immunother

Circulating PD-L1 is associated with T cell infiltration and predicts prognosis in patients with CRLM following hepatic resection.

Authors - Xiuxing Chen
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Guorong (Verified Customer) (05-23-2022)

excellent with 293T cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: 293T
Annexin A1 Antibody Western Blot validation (1:2000 dilution) in 293T (Cat no:21990-1-AP)