- Featured Product
- KD/KO Validated
Annexin A1 Polyclonal antibody
Annexin A1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 55018-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | C2C12 cell, HeLa cells, NIH/3T3 cells, HepG2 cells, C6 cells, K-562 cells |
| Positive IHC detected in | human colon tissue, human oesophagus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
55018-1-AP targets Annexin A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | annexin A1 |
| Calculated molecular weight | 40 kDa |
| Observed molecular weight | 39 kDa |
| GenBank accession number | NM_000700 |
| Gene symbol | Annexin A1 |
| Gene ID (NCBI) | 301 |
| RRID | AB_10860266 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes. The antibody is specific to ANXA1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Annexin A1 antibody 55018-1-AP | Download protocol |
| IHC protocol for Annexin A1 antibody 55018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Expert Rev Mol Diagn Co-overexpression of Hsp90-β and annexin A1 with a significantly positive correlation contributes to the diagnosis of lung cancer. | ||
J Cell Sci Platelet-derived microvesicles regulate vascular smooth muscle cell energy metabolism via PRKAA after intimal injury. | ||
J Proteomics iTRAQ-based comparative proteomic analysis of Vero cells infected with virulent and CV777 vaccine strain-like strains of porcine epidemic diarrhea virus. | ||
Mediators Inflamm Interaction between ANXA1 and GATA-3 in Immunosuppression of CD4(+) T Cells. | ||
J Exp Clin Cancer Res Upregulation of Hsp90-beta and annexin A1 correlates with poor survival and lymphatic metastasis in lung cancer patients. | ||
Am J Transl Res Elevated serum annexin A1 as potential diagnostic marker for lung cancer: a retrospective case-control study. |
