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KD/KO Validated

Annexin A11 Polyclonal antibody

Name: Annexin A11 Polyclonal antibody
Brand: Proteintech
SKU: 10479-2-AP

Annexin A11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10479-2-AP

Synonyms

ANXA11, ANX11, Annexin-11, Annexin XI, Annexin 11

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 10479-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10479-2-AP (Annexin A11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10479-2-AP
HeLa (WT and ANXA11 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 10479-2-AP incubated at 1:2000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IP experiment of HeLa using 10479-2-AP
HeLa lysates prepared and IP of ANXA11 performed using 1.0 μg of 10479-2-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IP experiment of HeLa using 10479-2-AP
IP result of anti-Annexin A11 (IP:10479-2-AP, 4ug; Detection:10479-2-AP 1:1000) with HeLa cells lysate 2320 ug.
IHC staining of human ovary tumor using 10479-2-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 10479-2-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 10479-2-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 10479-2-AP (Annexin A11 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 10479-2-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 10479-2-AP (Annexin A11 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 10479-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10479-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10479-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10479-2-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10479-2-AP (Annexin A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10479-2-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Annexin A11 antibody (10479-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 10479-2-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A11 antibody (10479-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 10479-2-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin A11 antibody (10479-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 10479-2-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, mouse uterus tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human ovary tumor tissue, human breast cancer tissue, human kidney tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 7 publications below
IF	See 4 publications below

Product Information

10479-2-AP targets Annexin A11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Annexin A11 fusion protein Ag0729 相同性解析による交差性が予測される生物種
Full Name	annexin A11
Calculated molecular weight	56 kDa
Observed molecular weight	56-60 kDa
GenBank accession number	BC007564
Gene symbol	Annexin A11
Gene ID (NCBI)	311
RRID	AB_2057171
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Annexin A11 (Anxa11) is one member of annexins that are Ca2+-regulated phospholipid-binding proteins. Annexin A11 plays an important role in cell division, differentiation, apoptosis, vesicle trafficking and Ca2+ signaling. Annexin A11 dysregulation is involved in the progression, drug-resistance, recurrence of systemic autoimmune disease and cancer.

Protocols

Product Specific Protocols
WB protocol for Annexin A11 antibody 10479-2-AP	Download protocol
IHC protocol for Annexin A11 antibody 10479-2-AP	Download protocol
IF protocol for Annexin A11 antibody 10479-2-AP	Download protocol
IP protocol for Annexin A11 antibody 10479-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC,IF	Sci Transl Med Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis. Authors - Bradley N Smith View Article
human	IHC,IF	Ann Neurol A Novel Multisystem Proteinopathy Caused by a Missense ANXA11 Variant. Authors - Tauana Bernardes Leoni View Article
mouse	WB	Oncotarget ANXA11 regulates the tumorigenesis, lymph node metastasis and 5-fluorouracil sensitivity of murine hepatocarcinoma Hca-P cells by targeting c-Jun. Authors - Shuqing Liu KD Validated View Article
mouse	WB	Biomed Pharmacother Annexin A11 knockdown inhibits in vitro proliferation and enhances survival of Hca-F cell via Akt2/FoxO1 pathway and MMP-9 expression. Authors - Shuqing Liu KD Validated View Article
human	WB,IHC	Neurobiol Aging Genetic screening of ANXA11 revealed novel mutations linked to amyotrophic lateral sclerosis. Authors - Elisa Teyssou View Article
human	IHC	Ann Clin Transl Neurol Adult-onset dominant muscular dystrophy in Greek families caused by Annexin A11 Authors - Mridul Johari View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Annexin A11 Polyclonal antibody

Annexin A11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 10479-2-AP

WB analysis of HeLa using 10479-2-AP

IP experiment of HeLa using 10479-2-AP

IP experiment of HeLa using 10479-2-AP

IHC staining of human ovary tumor using 10479-2-AP

IHC staining of human ovary tumor using 10479-2-AP

IHC staining of human kidney using 10479-2-AP

IHC staining of human kidney using 10479-2-AP

IHC staining of human breast cancer using 10479-2-AP

IHC staining of human breast cancer using 10479-2-AP

IHC staining of human lung cancer using 10479-2-AP

IHC staining of human lung cancer using 10479-2-AP

IF Staining of HeLa using 10479-2-AP

IF Staining of HeLa using 10479-2-AP

IF Staining of HeLa using 10479-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis.

A Novel Multisystem Proteinopathy Caused by a Missense ANXA11 Variant.

ANXA11 regulates the tumorigenesis, lymph node metastasis and 5-fluorouracil sensitivity of murine hepatocarcinoma Hca-P cells by targeting c-Jun.

Annexin A11 knockdown inhibits in vitro proliferation and enhances survival of Hca-F cell via Akt2/FoxO1 pathway and MMP-9 expression.

Genetic screening of ANXA11 revealed novel mutations linked to amyotrophic lateral sclerosis.

Adult-onset dominant muscular dystrophy in Greek families caused by Annexin A11