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Annexin A2 Monoclonal antibody

Name: Annexin A2 Monoclonal antibody
Brand: Proteintech
SKU: 66035-1-Ig

Annexin A2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

CloneNo.

1C1E12

Review Product

Cat no : 66035-1-Ig

Synonyms

ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis using 66035-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 66035-1-Ig
WB result of Annexin A2 antibody (66035-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A2 transfected HEK-293 cells.
WB analysis using 66035-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 66035-1-Ig
MCF7 cells were subjected to SDS PAGE followed by western blot with 66035-1-Ig (ANXA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 66035-1-Ig
human brain tissue were subjected to SDS PAGE followed by western blot with 66035-1-Ig (ANXA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 66035-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 66035-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66035-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 66035-1-Ig
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 66035-1-Ig
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of T-47D using 66035-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 66035-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of HeLa using 66035-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66035-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
FC experiment of HeLa using 66035-1-Ig
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Annexin A2 Monoclonal antibody (66035-1-Ig, Clone:1C1E12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 66035-1-Ig

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	K-562 cells, MCF-7 cells, human brain tissue, HepG2 cells, HEK-293 cells, A549 cells, HeLa cells, A431 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected in	human breast cancer tissue, human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	T-47D cells, HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:5000-1:20000
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 16 publications below
IHC	See 5 publications below
IF	See 9 publications below
IP	See 3 publications below
CoIP	See 4 publications below

Product Information

66035-1-Ig targets Annexin A2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	Annexin A2 fusion protein Ag16654 相同性解析による交差性が予測される生物種
Full Name	annexin A2
Calculated molecular weight	339 aa, 39 kDa
Observed molecular weight	39 kDa
GenBank accession number	BC016774
Gene symbol	Annexin A2
Gene ID (NCBI)	302
RRID	AB_11045659
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

Protocols

Product Specific Protocols
WB protocol for Annexin A2 antibody 66035-1-Ig	Download protocol
IHC protocol for Annexin A2 antibody 66035-1-Ig	Download protocol
IF protocol for Annexin A2 antibody 66035-1-Ig	Download protocol
FC protocol for Annexin A2 antibody 66035-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC,IF,IP	Oncogene Hypoxia-induced circADAMTS6 in a TDP43-dependent manner accelerates glioblastoma progression via ANXA2/ NF-κB pathway Authors - Shulin Zhao KD Validated View Article
human		Pharmacol Res Chip-DSF: A rapid screening strategy for drug protein targets. Authors - Zhao Cui View Article
human	WB, IF, IP	J Exp Clin Cancer Res Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma. Authors - Lu Deng View Article
human,mouse	WB,IHC,IF,CoIP	Biomed Pharmacother Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo. Authors - Lei Wang View Article
human	WB	Biosci Rep Role of Annexin A2 isoform2 on the aggregative growth of dermal papillae cells. Authors - Jing Gu KD Validated View Article
		Analyst An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines. Authors - Yu Yanyan Y View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Annexin A2 Monoclonal antibody

Annexin A2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 66035-1-Ig

WB analysis of HEK-293 using 66035-1-Ig

WB analysis using 66035-1-Ig

WB analysis of MCF-7 using 66035-1-Ig

WB analysis of human brain using 66035-1-Ig

WB analysis using 66035-1-Ig

IHC staining of human breast cancer using 66035-1-Ig

IHC staining of human breast cancer using 66035-1-Ig

IHC staining of human skin cancer using 66035-1-Ig

IHC staining of human skin cancer using 66035-1-Ig

IF Staining of T-47D using 66035-1-Ig

IF Staining of HeLa using 66035-1-Ig

FC experiment of HeLa using 66035-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Hypoxia-induced circADAMTS6 in a TDP43-dependent manner accelerates glioblastoma progression via ANXA2/ NF-κB pathway

Chip-DSF: A rapid screening strategy for drug protein targets.

Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma.

Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo.

Role of Annexin A2 isoform2 on the aggregative growth of dermal papillae cells.

An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.