Annexin A4 Polyclonal antibody

Annexin A4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10087-1-AP

Synonyms

ANXA4, ANX4, Annexin-4, Annexin IV, Annexin 4


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Tested Applications

Positive WB detected inHuH-7 cells, RAW 264.7 cells, C6
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10087-1-AP targets Annexin A4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin A4 fusion protein Ag0130 相同性解析による交差性が予測される生物種
Full Name annexin A4
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000182
Gene symbol Annexin A4
Gene ID (NCBI) 307
RRIDAB_2057474
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A4 (Anxa4) is one of the Ca2 +-regulated and phospholipid-binding annexin superfamily proteins, and is located at 2p13. Anxa4 has a potential role in diagnosis, prognosis, and treatment of certain cancers. Annexin A4 shares 45 to 59% identity with other members and possibly interacts with ATP. Annexin A4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. The Annexin A4 protein is expressed in a wide range of tissues, associated with polarized epithelial cells and is proposed to be involved in the regulation of chloride ions across the epithelial membrane.

Protocols

Product Specific Protocols
WB protocol for Annexin A4 antibody 10087-1-APDownload protocol
IHC protocol for Annexin A4 antibody 10087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Proteomics analyses of human optic nerve head astrocytes following biomechanical strain.

Authors - Rogers Ronan S RS
humanWB

Aging (Albany NY)

Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma.

Authors - Ya Peng
mouseWB

J Immunol

Pathogenic natural antibodies recognizing annexin IV are required to develop intestinal ischemia-reperfusion injury.

Authors - Kulik Liudmila L
humanWB

Mol Cell Proteomics

Proteomics Profiling of Autologous Blood and Semen Exosomes from HIV-infected and Uninfected Individuals Reveals Compositional and Functional Variabilities.

Authors - Hussein Kaddour
humanWB,IHC

Sci Rep

Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification.

Authors - Kewu Jiang
humanWB

PLoS One

Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways.

Authors - Qi Ying

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yuan (Verified Customer) (09-30-2019)

The antibody is working very well on HeLa cells.

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa