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  • Featured Product
  • KD/KO Validated

Annexin V Polyclonal antibody

Annexin V Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 11060-1-AP

Synonyms

Annexin A5, ANXA5, ANX5, Annexin-5, Annexin 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Annexin V Polyclonal antibody (11060-1-AP)

    WB analysis using 11060-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Annexin V Polyclonal antibody (11060-1-AP)

    WB analysis using 11060-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig heart tissue using Annexin V Polyclonal antibody (11060-1-AP)

    WB analysis of pig heart using 11060-1-AP

    pig heart tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using Annexin V Polyclonal antibody (11060-1-AP)

    WB analysis of pig liver using 11060-1-AP

    pig liver tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using Annexin V Polyclonal antibody (11060-1-AP)

    WB analysis of NIH/3T3 using 11060-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lung tissue using Annexin V Polyclonal antibody (11060-1-AP)

    WB analysis of human lung using 11060-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using Annexin V Polyclonal antibody (11060-1-AP)

    WB analysis of human heart using 11060-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Annexin V Polyclonal antibody (11060-1-AP)

    WB analysis of human placenta using 11060-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using Annexin V Polyclonal antibody (11060-1-AP)

    WB analysis of human skeletal muscle using 11060-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using Annexin V Polyclonal antibody (11060-1-AP)

    WB analysis of A431 using 11060-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Annexin V Polyclonal antibody (11060-1-AP)

    WB analysis of HepG2 using 11060-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Annexin V Polyclonal antibody (11060-1-AP)

    IP experiment of HeLa using 11060-1-AP

    IP result of anti-Annexin V (IP:11060-1-AP, 3ug; Detection:11060-1-AP 1:500) with HeLa cells lysate 3000ug.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using Annexin V Polyclonal antibody (11060-1-AP)

    IP experiment of mouse liver using 11060-1-AP

    IP result of anti-Annexin V (IP:11060-1-AP, 4ug; Detection:11060-1-AP 1:600) with mouse liver tissue lysate 7000ug.

  • Immunohistochemistry (IHC) staining of human spleen tissue using Annexin V Polyclonal antibody (11060-1-AP)

    IHC staining of human spleen using 11060-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11060-1-AP (Annexin V antibody at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using Annexin V Polyclonal antibody (11060-1-AP)

    IHC staining of human spleen using 11060-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11060-1-AP (Annexin V antibody at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Annexin V Polyclonal antibody (11060-1-AP)

    IHC staining of human placenta using 11060-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11060-1-AP (Annexin V antibody at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Annexin V Polyclonal antibody (11060-1-AP)

    IHC staining of human placenta using 11060-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11060-1-AP (Annexin V antibody at dilution of 1:400 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Annexin V Polyclonal antibody (11060-1-AP)

    IF Staining of HeLa using 11060-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin V antibody (11060-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Annexin V Polyclonal antibody (11060-1-AP)

    IF Staining of HeLa using 11060-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Annexin V antibody (11060-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using Annexin V Polyclonal antibody (11060-1-AP)

    FC experiment of HeLa using 11060-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Annexin V Polyclonal antibody (11060-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHeLa cells, A431 cells, C6 cells, HepG2 cells, human heart tissue, human lung tissue, human placenta tissue, human skeletal muscle tissue, NIH/3T3 cells, pig heart tissue, pig liver tissue, HEK-293T cells, K-562 cells, NIH?3T3 cells, C2C12 cells
Positive IP detected inHeLa cells, mouse liver tissue
Positive IHC detected inhuman spleen tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11060-1-AP targets Annexin V in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin V fusion protein Ag1538 相同性解析による交差性が予測される生物種
Full Name annexin A5
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC018671
Gene symbol Annexin V
Gene ID (NCBI) 308
RRIDAB_2057585
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

Protocols

Product Specific Protocols
WB protocol for Annexin V antibody 11060-1-APDownload protocol
IHC protocol for Annexin V antibody 11060-1-APDownload protocol
IF protocol for Annexin V antibody 11060-1-APDownload protocol
IP protocol for Annexin V antibody 11060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Matrix Biol

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Authors - Emna Ouni
View Article
human,mouseWB,IHC,IF

Cell Death Dis

Annexin A5 regulates hepatocarcinoma malignancy via CRKI/II-DOCK180-RAC1 integrin and MEK-ERK pathways.

Authors - Xujuan Sun
  • KD Validated
View Article
humanIHC

Front Cell Dev Biol

Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis.

Authors - Mansheng Li
View Article

Am J Physiol Heart Circ Physiol

Epidermal growth factor receptor inhibition prevents vascular calcifying extracellular vesicle biogenesis

Authors - Amirala Bakhshian Nik
View Article
mouseWB

J Proteome Res

Enrichment of Neurodegenerative Microglia Signature in Brain-Derived Extracellular Vesicles Isolated from Alzheimer's Disease Mouse Models.

Authors - Satoshi Muraoka
View Article
humanWB

Endocrinology

Reassessment of the Proteomic Composition and Function of Extracellular Vesicles in the Seminal Plasma.

Authors - Hanshu Wang
View Article