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KD/KO Validated

Annexin V Polyclonal antibody

Name: Annexin V Polyclonal antibody
Brand: Proteintech
SKU: 11060-1-AP

Annexin V Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11060-1-AP

Synonyms

Annexin A5, ANXA5, ANX5, Annexin-5, Annexin 5

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 11060-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 11060-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.
WB analysis of pig heart using 11060-1-AP
pig heart tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of pig liver using 11060-1-AP
pig liver tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 11060-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 11060-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 11060-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 11060-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11060-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 11060-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11060-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 11060-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11060-1-AP
IP result of anti-Annexin V (IP:11060-1-AP, 3ug; Detection:11060-1-AP 1:500) with HeLa cells lysate 3000ug.
IP experiment of mouse liver using 11060-1-AP
IP result of anti-Annexin V (IP:11060-1-AP, 4ug; Detection:11060-1-AP 1:600) with mouse liver tissue lysate 7000ug.
IHC staining of human spleen using 11060-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11060-1-AP (Annexin V antibody at dilution of 1:400 (under 10x lens).
IHC staining of human spleen using 11060-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11060-1-AP (Annexin V antibody at dilution of 1:400 (under 40x lens).
IHC staining of human placenta using 11060-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11060-1-AP (Annexin V antibody at dilution of 1:400 (under 10x lens).
IHC staining of human placenta using 11060-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11060-1-AP (Annexin V antibody at dilution of 1:400 (under 40x lens).
IF Staining of HeLa using 11060-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin V antibody (11060-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 11060-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Annexin V (11060-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 11060-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, A431 cells, C6 cells, HEK-293 cells, HepG2 cells, human heart tissue, human lung tissue, human placenta tissue, human skeletal muscle tissue, NIH/3T3 cells, pig heart tissue, pig liver tissue, HEK-293T cells, K-562 cells, NIH?3T3 cells, C2C12 cells
Positive IP detected in	HeLa cells, mouse liver tissue
Positive IHC detected in	human spleen tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 18 publications below
IHC	See 7 publications below
IF	See 9 publications below

Product Information

11060-1-AP targets Annexin V in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Annexin V fusion protein Ag1538 相同性解析による交差性が予測される生物種
Full Name	annexin A5
Calculated molecular weight	36 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC018671
Gene symbol	Annexin V
Gene ID (NCBI)	308
RRID	AB_2057585
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

Protocols

Product Specific Protocols
WB protocol for Annexin V antibody 11060-1-AP	Download protocol
IHC protocol for Annexin V antibody 11060-1-AP	Download protocol
IF protocol for Annexin V antibody 11060-1-AP	Download protocol
IP protocol for Annexin V antibody 11060-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. Authors - Emna Ouni View Article
human,mouse	WB,IHC,IF	Cell Death Dis Annexin A5 regulates hepatocarcinoma malignancy via CRKI/II-DOCK180-RAC1 integrin and MEK-ERK pathways. Authors - Xujuan Sun KD Validated View Article
human	IHC	Front Cell Dev Biol Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis. Authors - Mansheng Li View Article
		Am J Physiol Heart Circ Physiol Epidermal growth factor receptor inhibition prevents vascular calcifying extracellular vesicle biogenesis Authors - Amirala Bakhshian Nik View Article
mouse	WB	J Proteome Res Enrichment of Neurodegenerative Microglia Signature in Brain-Derived Extracellular Vesicles Isolated from Alzheimer's Disease Mouse Models. Authors - Satoshi Muraoka View Article
human	WB	Endocrinology Reassessment of the Proteomic Composition and Function of Extracellular Vesicles in the Seminal Plasma. Authors - Hanshu Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Annexin V Polyclonal antibody

Annexin V Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 11060-1-AP

WB analysis using 11060-1-AP

WB analysis of pig heart using 11060-1-AP

WB analysis of pig liver using 11060-1-AP

WB analysis of human lung using 11060-1-AP

WB analysis of human heart using 11060-1-AP

WB analysis of human placenta using 11060-1-AP

WB analysis of human skeletal muscle using 11060-1-AP

WB analysis of HEK-293 using 11060-1-AP

WB analysis of A431 using 11060-1-AP

WB analysis of HepG2 using 11060-1-AP

WB analysis of NIH/3T3 using 11060-1-AP

IP experiment of HeLa using 11060-1-AP

IP experiment of mouse liver using 11060-1-AP

IHC staining of human spleen using 11060-1-AP

IHC staining of human spleen using 11060-1-AP

IHC staining of human placenta using 11060-1-AP

IHC staining of human placenta using 11060-1-AP

IF Staining of HeLa using 11060-1-AP

FC experiment of HeLa using 11060-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Annexin A5 regulates hepatocarcinoma malignancy via CRKI/II-DOCK180-RAC1 integrin and MEK-ERK pathways.

Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis.

Epidermal growth factor receptor inhibition prevents vascular calcifying extracellular vesicle biogenesis

Enrichment of Neurodegenerative Microglia Signature in Brain-Derived Extracellular Vesicles Isolated from Alzheimer's Disease Mouse Models.

Reassessment of the Proteomic Composition and Function of Extracellular Vesicles in the Seminal Plasma.

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