Featured Product
KD/KO Validated

Annexin V Polyclonal antibody

Name: Annexin V Polyclonal antibody
Brand: Proteintech
SKU: 11060-1-AP

Annexin V Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11060-1-AP

Synonyms

Annexin A5, ANXA5, ANX5, Annexin-5, Annexin 5

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 11060-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 11060-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 11060-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 11060-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 11060-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 11060-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 11060-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 11060-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11060-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of pig heart using 11060-1-AP
pig heart tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of pig liver using 11060-1-AP
pig liver tissue were subjected to SDS PAGE followed by western blot with 11060-1-AP (Annexin V antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11060-1-AP
IP result of anti-Annexin V (IP:11060-1-AP, 3ug; Detection:11060-1-AP 1:500) with HeLa cells lysate 3000ug.
IP experiment of mouse liver using 11060-1-AP
IP result of anti-Annexin V (IP:11060-1-AP, 4ug; Detection:11060-1-AP 1:600) with mouse liver tissue lysate 7000ug.
IHC staining of human spleen using 11060-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11060-1-AP (Annexin V antibody at dilution of 1:400 (under 10x lens).
IHC staining of human spleen using 11060-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11060-1-AP (Annexin V antibody at dilution of 1:400 (under 40x lens).
IHC staining of human placenta using 11060-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11060-1-AP (Annexin V antibody at dilution of 1:400 (under 10x lens).
IHC staining of human placenta using 11060-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11060-1-AP (Annexin V antibody at dilution of 1:400 (under 40x lens).
IF Staining of HeLa using 11060-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin V antibody (11060-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 11060-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Annexin V Polyclonal antibody (11060-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

-/ -

Cat No. 11060-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, A431 cells, C6 cells, HepG2 cells, human heart tissue, human lung tissue, human placenta tissue, human skeletal muscle tissue, NIH/3T3 cells, pig heart tissue, pig liver tissue, HEK-293T cells, K-562 cells, NIH?3T3 cells, C2C12 cells
Positive IP detected in	HeLa cells, mouse liver tissue
Positive IHC detected in	human spleen tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 18 publications below
IHC	See 7 publications below
IF	See 9 publications below

Product Information

11060-1-AP targets Annexin V in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Annexin V fusion protein Ag1538 相同性解析による交差性が予測される生物種
Full Name	annexin A5
Calculated molecular weight	36 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC018671
Gene symbol	Annexin V
Gene ID (NCBI)	308
RRID	AB_2057585
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

Protocols

Product Specific Protocols
WB protocol for Annexin V antibody 11060-1-AP	Download protocol
IHC protocol for Annexin V antibody 11060-1-AP	Download protocol
IF protocol for Annexin V antibody 11060-1-AP	Download protocol
IP protocol for Annexin V antibody 11060-1-AP	Download protocol
FC protocol for Annexin V antibody 11060-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. Authors - Emna Ouni View Article
human,mouse	WB,IHC,IF	Cell Death Dis Annexin A5 regulates hepatocarcinoma malignancy via CRKI/II-DOCK180-RAC1 integrin and MEK-ERK pathways. Authors - Xujuan Sun KD Validated View Article
human	IHC	Front Cell Dev Biol Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis. Authors - Mansheng Li View Article
		Am J Physiol Heart Circ Physiol Epidermal growth factor receptor inhibition prevents vascular calcifying extracellular vesicle biogenesis Authors - Amirala Bakhshian Nik View Article
mouse	WB	J Proteome Res Enrichment of Neurodegenerative Microglia Signature in Brain-Derived Extracellular Vesicles Isolated from Alzheimer's Disease Mouse Models. Authors - Satoshi Muraoka View Article
human	WB	Endocrinology Reassessment of the Proteomic Composition and Function of Extracellular Vesicles in the Seminal Plasma. Authors - Hanshu Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Annexin V Polyclonal antibody

Annexin V Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 11060-1-AP

WB analysis using 11060-1-AP

WB analysis of NIH/3T3 using 11060-1-AP

WB analysis of human lung using 11060-1-AP

WB analysis of human heart using 11060-1-AP

WB analysis of human placenta using 11060-1-AP

WB analysis of human skeletal muscle using 11060-1-AP

WB analysis of A431 using 11060-1-AP

WB analysis of HepG2 using 11060-1-AP

WB analysis of pig heart using 11060-1-AP

WB analysis of pig liver using 11060-1-AP

IP experiment of HeLa using 11060-1-AP

IP experiment of mouse liver using 11060-1-AP

IHC staining of human spleen using 11060-1-AP

IHC staining of human spleen using 11060-1-AP

IHC staining of human placenta using 11060-1-AP

IHC staining of human placenta using 11060-1-AP

IF Staining of HeLa using 11060-1-AP

FC experiment of HeLa using 11060-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Annexin A5 regulates hepatocarcinoma malignancy via CRKI/II-DOCK180-RAC1 integrin and MEK-ERK pathways.

Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis.

Epidermal growth factor receptor inhibition prevents vascular calcifying extracellular vesicle biogenesis

Enrichment of Neurodegenerative Microglia Signature in Brain-Derived Extracellular Vesicles Isolated from Alzheimer's Disease Mouse Models.

Reassessment of the Proteomic Composition and Function of Extracellular Vesicles in the Seminal Plasma.

Related Products

CFTR Polyclonal antibody

Annexin A4 Polyclonal antibody

Annexin A1 Polyclonal antibody

Annexin A1 Polyclonal antibody

Annexin A2 Polyclonal antibody

Annexin VI Polyclonal antibody

Annexin A3 Polyclonal antibody