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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ANXA5 Recombinant antibody

ANXA5 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240621D1

Cat no : 83929-1-RR

Synonyms

Annexin A5, Annexin-5, Annexin V, Annexin 5, Anchorin CII

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANXA5 Recombinant antibody (83929-1-RR)

    WB analysis using 83929-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83929-1-RR (ANXA5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83929-1-RR

    Affinity and Kinetic Characterization of 83929-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83929-1-RR against Human ANXA5 were performed. The affinity constant is 13.4 nM.

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Tested Applications

Positive WB detected inHEK-293T cells, HepG2 cells, K-562 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83929-1-RR is ANXA5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ANXA5 fusion protein Ag0741 相同性解析による交差性が予測される生物種
Full Name annexin A5
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC001429
Gene symbol Annexin V
Gene ID (NCBI) 308
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

Protocols

Product Specific Protocols
WB protocol for ANXA5 antibody 83929-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols