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Annexin VI Polyclonal antibody
Annexin VI Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 12542-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, human brain tissue, mouse brain tissue, mouse placenta tissue, mouse kidney tissue, A549 cells, Jurkat cells |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | human colon tissue, human kidney tissue, human normal colon, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 10 publications below |
IHC | See 2 publications below |
IF | See 3 publications below |
IP | See 1 publications below |
Product Information
12542-1-AP targets Annexin VI in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Annexin VI fusion protein Ag3263 相同性解析による交差性が予測される生物種 |
Full Name | annexin A6 |
Calculated molecular weight | 673 aa, 76 kDa |
Observed molecular weight | 67 kDa |
GenBank accession number | BC017046 |
Gene symbol | Annexin VI |
Gene ID (NCBI) | 309 |
RRID | AB_2057753 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Annexin VI antibody 12542-1-AP | Download protocol |
IHC protocol for Annexin VI antibody 12542-1-AP | Download protocol |
IF protocol for Annexin VI antibody 12542-1-AP | Download protocol |
IP protocol for Annexin VI antibody 12542-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Extracell Vesicles Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion-exchange method. | ||
Oncotarget CD300A promotes tumor progression by PECAM1, ADCY7 and AKT pathway in acute myeloid leukemia. | ||
Front Cell Dev Biol Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging. | ||
Proteome Sci A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis. | ||
Onco Targets Ther UNBS5162 inhibits proliferation of human retinoblastoma cells by promoting cell apoptosis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Gaurav (Verified Customer) (02-29-2024) | Very good antibody with expected band at appropriate position. Works well in TBST, 5% Skimmed milk and 3% BSA in TBST
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