Validation Data Gallery

Filter: AllWBIPIHCFC (Intra)

WB analysis using 10154-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10154-2-AP (Annexin A7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 10154-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10154-2-AP (Annexin A7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 10154-2-AP
L02 cells were subjected to SDS PAGE followed by western blot with 10154-2-AP (Annexin VII antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 10154-2-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 10154-2-AP (Annexin VII antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of U-87 MG using 10154-2-AP
IP result of anti-Annexin A7 (IP:10154-2-AP, 4ug; Detection:10154-2-AP 1:8000) with U-87 MG cells lysate 1160 ug.
IP experiment of mouse heart using 10154-2-AP
IP result of anti-Annexin A7 (IP:10154-2-AP, 4ug; Detection:10154-2-AP 1:800) with mouse heart tissue lysate 3200ug.
IHC staining of human pancreas using 10154-2-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10154-2-AP (Annexin VII antibody at dilution of 1:50 (under 40x lens).
IHC staining of human stomach using 10154-2-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 10154-2-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 10154-2-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 10154-2-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 10154-2-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 10154-2-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 10154-2-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 10154-2-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 10154-2-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 10154-2-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10154-2-AP (Annexin A7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 10154-2-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10154-2-AP (Annexin VII antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 10154-2-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10154-2-AP (Annexin VII antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of SH-SY5Y using 10154-2-AP
1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Annexin A7 (10154-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

Annexin A7 Polyclonal antibody

Annexin A7 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 10154-2-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Synonyms

ANXA7, Annexin 7, Annexin VII, Annexin-7, ANX7

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, Jurkat cells, mouse lung tissue, L02 cells, mouse brain tissue, mouse heart tissue, HeLa cells, RAW 264.7 cells, rat brain tissue
Positive IP detected in	U-87 MG cells, mouse heart tissue
Positive IHC detected in	mouse heart tissue, human pancreas tissue, human prostate cancer tissue, human stomach tissue, human stomach cancer tissue, mouse stomach tissue, rat stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 10 publications below
IHC	See 4 publications below
IF	See 4 publications below
IP	See 1 publications below

Product Information

10154-2-AP targets Annexin A7 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0206 Product name: Recombinant human ANXA7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 236-466 aa of BC002632 Sequence: PTYYDAWSLRKAMQGAGTQERVLIEILCTRTNQEIREIVRCYQSEFGRDLEKDIRSDTSGHFERLLVSMCQGNRDENQSINHQMAQEDAQRLYQAGEGRLGTDESCFNMILATRSFPQLRATMEAYSRMANRDLLSSVSREFSGYVESGLKTILQCALNRPAFFAERLYYAMKGAGTDDSTLVRIVVTRSEIDLVQIKQMFAQMYQKTLGTMIAGDTSGDYRRLLLAIVGQ 相同性解析による交差性が予測される生物種
Full Name	annexin A7
Calculated molecular weight	50 kDa
Observed molecular weight	47 kDa, 51 kDa
GenBank accession number	BC002632
Gene Symbol	Annexin A7
Gene ID (NCBI)	310
RRID	AB_2227386
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P20073
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Annexin A7 (Anx7) belongs to a ubiquitous and relatively abundant family of Ca2+-dependent membrane-binding proteins, which are thought to be involved in multiple aspects of cell biology including membrane trafficking, mediation of cell-matrix interactions and membrane organization within cells. Anx7, migrated as a 50 kDa protein in SDS-PAGE, has been proposed to function in the fusion of vesicles, acting as a Ca++ channel and as Ca++ -activated GTPase, thus inducing Ca++ /GTP-dependent secretory events.

Protocols

Product Specific Protocols
IHC protocol for Annexin A7 antibody 10154-2-AP	Download protocol
IP protocol for Annexin A7 antibody 10154-2-AP	Download protocol
WB protocol for Annexin A7 antibody 10154-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. Authors - Emna Ouni View Article
human	WB	Am J Physiol Endocrinol Metab Differential expression of the regulated catecholamine secretory pathway in different hereditary forms of pheochromocytoma. Authors - Eisenhofer Graeme G View Article
human	WB,IF	J Proteome Res Quantitative Mitochondrial Proteomics Reveals ANXA7 as a Crucial Factor in Mitophagy. Authors - Kun Meng KD Validated View Article
human	IHC	Am J Transl Res Downregulation of annexin A7 decreases proliferation, migration, and invasion of gastric cancer cells by reducing matrix metalloproteinase 1 and 9 expression. Authors - Hu-Fang Yuan View Article
mouse	WB	Biofactors Ezrin as a possible diagnostic and/or prognostic biomarker in mice lymphatic metastatic hepatocellular carcinoma in vivo. Authors - Ahmed Musa Hago Bakheet View Article
human	WB	FEBS Lett Annexins A1 and A2 are recruited to larger lysosomal injuries independently of ESCRTs to promote repair. Authors - Willa Wen-You Yim View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10154-2-AP

WB analysis using 10154-2-AP

WB analysis of L02 using 10154-2-AP

WB analysis of mouse lung using 10154-2-AP

IP experiment of U-87 MG using 10154-2-AP

IP experiment of mouse heart using 10154-2-AP

IHC staining of human pancreas using 10154-2-AP

IHC staining of human stomach using 10154-2-AP

IHC staining of human stomach using 10154-2-AP

IHC staining of human stomach cancer using 10154-2-AP

IHC staining of human stomach cancer using 10154-2-AP

IHC staining of rat stomach using 10154-2-AP

IHC staining of rat stomach using 10154-2-AP

IHC staining of mouse stomach using 10154-2-AP

IHC staining of mouse stomach using 10154-2-AP

IHC staining of mouse heart using 10154-2-AP

IHC staining of mouse lung using 10154-2-AP

IHC staining of human prostate cancer using 10154-2-AP

IHC staining of human prostate cancer using 10154-2-AP

FC experiment of SH-SY5Y using 10154-2-AP

Annexin A7 Polyclonal antibody

Annexin A7 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Differential expression of the regulated catecholamine secretory pathway in different hereditary forms of pheochromocytoma.

Quantitative Mitochondrial Proteomics Reveals ANXA7 as a Crucial Factor in Mitophagy.

Downregulation of annexin A7 decreases proliferation, migration, and invasion of gastric cancer cells by reducing matrix metalloproteinase 1 and 9 expression.

Ezrin as a possible diagnostic and/or prognostic biomarker in mice lymphatic metastatic hepatocellular carcinoma in vivo.

Annexins A1 and A2 are recruited to larger lysosomal injuries independently of ESCRTs to promote repair.