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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VAP1 Polyclonal antibody

VAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14365-1-AP

Synonyms

AOC3, HPAO, EC:1.4.3.21, Copper amine oxidase, Amine oxidase copper-containing 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VAP1 Polyclonal antibody (14365-1-AP)

    WB analysis using 14365-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14365-1-AP (VAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using VAP1 Polyclonal antibody (14365-1-AP)

    WB analysis using 14365-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14365-1-AP (VAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using VAP1 Polyclonal antibody (14365-1-AP)

    IHC staining of human appendicitis using 14365-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14365-1-AP (VAP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using VAP1 Polyclonal antibody (14365-1-AP)

    IHC staining of human appendicitis using 14365-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14365-1-AP (VAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using VAP1 Polyclonal antibody (14365-1-AP)

    IHC staining of human colon using 14365-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14365-1-AP (VAP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VAP1 Polyclonal antibody (14365-1-AP)

    IHC staining of human lung cancer using 14365-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VAP1 Polyclonal antibody (14365-1-AP)

    IHC staining of human lung cancer using 14365-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using VAP1 Polyclonal antibody (14365-1-AP)

    IHC staining of human small intestine using 14365-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using VAP1 Polyclonal antibody (14365-1-AP)

    IHC staining of human small intestine using 14365-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inmouse lung tissue, mouse adipose tissue, rat lung tissue, mouse stomach tissue
Positive IHC detected inhuman appendicitis tissue, human lung cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

14365-1-AP targets VAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAP1 fusion protein Ag5683 相同性解析による交差性が予測される生物種
Full Name amine oxidase, copper containing 3 (vascular adhesion protein 1)
Calculated molecular weight 85 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC050549
Gene symbol AOC3
Gene ID (NCBI) 8639
RRIDAB_2058043
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AOC3(membrane primary amine oxidase) is also name as copper amine oxidase, HPAO, SSAO, VAP1 and belongs to the copper/topaquinone oxidase family. It is expressed on the surface of endothelial cells and is involved in leukocyte trafficking between blood and tissues under physiologic and pathologic conditions (PMID:17947691). AOC3 is highly expressed in adipocytes and smooth muscle cells and it can be N- and O-glycosylated (PMID:16046623). AOC3 has molecular mass of 70-90kD, and can exsit as a homodimer(165-185kD) and trimer(240~260kD) (PMID:10595925, 8625974).

Protocols

Product Specific Protocols
WB protocol for VAP1 antibody 14365-1-APDownload protocol
IHC protocol for VAP1 antibody 14365-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Cell Mol Med

Single-cell transcriptional profiling of human carotid plaques reveals a subpopulation of endothelial cells associated with stroke incidences.

Authors - Fengchan Li
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Hua (Verified Customer) (10-21-2020)

Right size band and Clean background, but with relatively weak signal.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse liver lysate
VAP1 Antibody Western Blot validation (1:1000 dilution) in mouse liver lysate (Cat no:14365-1-AP)