Validation Data Gallery

Filter: AllWBIHC

WB analysis using 14365-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14365-1-AP (VAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 14365-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14365-1-AP (VAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human appendicitis using 14365-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14365-1-AP (VAP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 14365-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14365-1-AP (VAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 14365-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14365-1-AP (VAP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 14365-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 14365-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human small intestine using 14365-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 10x lens).
IHC staining of human small intestine using 14365-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14365-1-AP (VAP1 antibody at dilution of 1:200 (under 40x lens).

VAP1 Polyclonal antibody

VAP1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14365-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

AOC3, HPAO, EC:1.4.3.21, Copper amine oxidase, Amine oxidase copper-containing 3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse lung tissue, mouse adipose tissue, rat lung tissue, mouse stomach tissue
Positive IHC detected in	human appendicitis tissue, human lung cancer tissue, human small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHC	See 1 publications below

Product Information

14365-1-AP targets VAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VAP1 fusion protein Ag5683 相同性解析による交差性が予測される生物種
Full Name	amine oxidase, copper containing 3 (vascular adhesion protein 1)
Calculated molecular weight	85 kDa
Observed molecular weight	85-90 kDa
GenBank accession number	BC050549
Gene Symbol	AOC3
Gene ID (NCBI)	8639
RRID	AB_2058043
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16853
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

AOC3(membrane primary amine oxidase) is also name as copper amine oxidase, HPAO, SSAO, VAP1 and belongs to the copper/topaquinone oxidase family. It is expressed on the surface of endothelial cells and is involved in leukocyte trafficking between blood and tissues under physiologic and pathologic conditions (PMID:17947691). AOC3 is highly expressed in adipocytes and smooth muscle cells and it can be N- and O-glycosylated (PMID:16046623). AOC3 has molecular mass of 70-90kD, and can exsit as a homodimer(165-185kD) and trimer(240~260kD) (PMID:10595925, 8625974).

Protocols

Product Specific Protocols
WB protocol for VAP1 antibody 14365-1-AP	Download protocol
IHC protocol for VAP1 antibody 14365-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	J Cell Mol Med Single-cell transcriptional profiling of human carotid plaques reveals a subpopulation of endothelial cells associated with stroke incidences. Authors - Fengchan Li View Article

Target

Host

Species Reactivity