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Validation Data Gallery

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  • Western Blot (WB) analysis of human ileum tissue using AOC3 Monoclonal antibody (66834-1-Ig)

    WB analysis of human ileum using 66834-1-Ig (same clone as 66834-1-PBS)

    human ileum tissue were subjected to SDS PAGE followed by western blot with 66834-1-Ig (AOC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AOC3 Monoclonal antibody (66834-1-Ig)

    IHC staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AOC3 Monoclonal antibody (66834-1-Ig)

    IHC staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AOC3 Monoclonal antibody (66834-1-Ig)

    IHC staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AOC3 Monoclonal antibody (66834-1-Ig)

    IHC staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66834-1-Ig (AOC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using AOC3 Monoclonal antibody (66834-1-Ig)

    IF Staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using AOC3 antibody (66834-1-Ig, Clone: 2A6G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using AOC3 Monoclonal antibody (66834-1-Ig)

    IF Staining of human liver cancer using 66834-1-Ig (same clone as 66834-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using AOC3 antibody (66834-1-Ig, Clone: 2A6G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66834-1-PBS in a different storage buffer formulation.

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AOC3 Monoclonal antibody, PBS Only

AOC3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66834-1-PBS
Clone No.2A6G3

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

AOC3, Copper amine oxidase, HPAO, Membrane primary amine oxidase, SSAO, VAP 1, VAP1, Vascular adhesion protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66834-1-PBS targets AOC3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AOC3 fusion protein Ag5908 相同性解析による交差性が予測される生物種
Full Name amine oxidase, copper containing 3 (vascular adhesion protein 1)
Calculated molecular weight 85 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC050549
Gene Symbol AOC3
Gene ID (NCBI) 8639
RRIDAB_2882177
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16853
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

AOC3(membrane primary amine oxidase) is also name as copper amine oxidase, HPAO, SSAO, VAP1 and belongs to the copper/topaquinone oxidase family. It is expressed on the surface of endothelial cells and is involved in leukocyte trafficking between blood and tissues under physiologic and pathologic conditions (PMID:17947691). AOC3 is highly expressed in adipocytes and smooth muscle cells and it can be N- and O-glycosylated (PMID:16046623). AOC3 has molecular mass of 70-90kD, and can exsit as a homodimer(165-185kD) and trimer(240~260kD) (PMID:10595925, 8625974).