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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACOX1 Recombinant antibody (83731-1-RR)

    WB analysis using 83731-1-RR (same clone as 83731-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83731-1-RR (ACOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83731-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00709-3

    Sandwich ELISA standard curve of MP00709-3

    Sandwich ELISA standard curve of MP00709-3, Human AOX Recombinant Matched Antibody Pair - PBS only. 83731-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1405. 83731-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Sample test of MP00709-3

    Sample test of MP00709-3

    K-562 and U-251 cell lysates were measured. The human AOX concentration of detected samples was determined to be 158.79 ng/mL (based on a 2.1 mg/mL extract load) in K-562 cell lyaste and 345.46 ng/mL (based on a 1.6 mg/mL extract load) in U-251 cell lysate.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using ACOX1 Recombinant antibody (83731-1-RR)

    IHC staining of human ovarian cancer using 83731-1-RR (same clone as 83731-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83731-1-RR (ACOX1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83731-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ACOX1 Recombinant antibody (83731-1-RR)

    IF Staining of HepG2 using 83731-1-RR (same clone as 83731-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AOX antibody (83731-1-RR, Clone: 240754D3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83731-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00709-3

    Cytometric bead array standard curve of MP00709-3

    Cytometric bead array standard curve of MP00709-3, AOX Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83731-1-PBS. Detection antibody: 83731-2-PBS. Standard: Ag1405. Range: 0.78-50 ng/mL.

  • Affinity and Kinetic Characterization of 83731-1-RR

    Affinity and Kinetic Characterization of 83731-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83731-1-RR against Human ACOX1 were performed. The affinity constant is 0.777 nM.

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ACOX1 Recombinant antibody, PBS Only (Capture)

ACOX1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83731-1-PBS
Clone No.240754D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1, ACOX

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83731-1-PBS targets ACOX1 as part of a matched antibody pair:

MP00709-3: 83731-1-PBS capture and 83731-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACOX1 fusion protein Ag1405 相同性解析による交差性が予測される生物種
Full Name acyl-Coenzyme A oxidase 1, palmitoyl
Calculated molecular weight 74 kDa
Observed molecular weight70-74 kDa, 22 kDa
GenBank accession numberBC010425
Gene Symbol ACOX1
Gene ID (NCBI) 51
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15067
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).