Featured Product
KD/KO Validated

Aldehyde oxidase Polyclonal antibody

Name: Aldehyde oxidase Polyclonal antibody
Brand: Proteintech
SKU: 19495-1-AP

Aldehyde oxidase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 19495-1-AP

Synonyms

EC:1.2.3.1, EC:1.17.3.-, Azaheterocycle hydroxylase, AOX1, AOX 1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 19495-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 19495-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 19495-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 19495-1-AP
IP result of anti-Aldehyde oxidase (IP:19495-1-AP, 4ug; Detection:19495-1-AP 1:300) with mouse lung tissue lysate 4000ug.
IHC staining of human hepatocirrhosis using 19495-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hepatocirrhosis using 19495-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 19495-1-AP
Immunofluorescent analysis of HepG2 cells, using AOX1 antibody 19495-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

-/ -

Cat No. 19495-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, mouse lung tissue, mouse pancreas tissue
Positive IP detected in	mouse lung tissue
Positive IHC detected in	human hepatocirrhosis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 10 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

19495-1-AP targets Aldehyde oxidase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity	human, mouse, rat, canine
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	aldehyde oxidase 1
Calculated molecular weight	148 kDa
Observed molecular weight	148 kDa
GenBank accession number	NM_001159
Gene symbol	AOX1
Gene ID (NCBI)	316
RRID	AB_10640579
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Aldehyde oxidase antibody 19495-1-AP	Download protocol
IHC protocol for Aldehyde oxidase antibody 19495-1-AP	Download protocol
IF protocol for Aldehyde oxidase antibody 19495-1-AP	Download protocol
IP protocol for Aldehyde oxidase antibody 19495-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	EMBO Rep Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice. Authors - Laura M Zidek View Article
human	WB,IHC	Oncogene Epigenetic loss of AOX1 expression via EZH2 leads to metabolic deregulations and promotes bladder cancer progression. Authors - Venkatrao Vantaku KD Validated View Article
human	IF	Am J Obstet Gynecol Evidence that fetal death is associated with placental aging. Authors - Kaushik Maiti View Article
mouse	WB	Free Radic Biol Med PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase. Authors - Xue Chen View Article
human	WB	Front Immunol Insight of a Metabolic Prognostic Model to Identify Tumor Environment and Drug Vulnerability for Lung Adenocarcinoma. Authors - Shun-Li Peng View Article
human	WB	Cell Signal Aldehyde oxidase 1 promotes gallbladder carcinogenesis through ROS-mediated activation of the Wnt/β-catenin pathway Authors - Ziheng Yuan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Aldehyde oxidase Polyclonal antibody

Aldehyde oxidase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 19495-1-AP

WB analysis of mouse lung using 19495-1-AP

WB analysis of mouse pancreas using 19495-1-AP

IP experiment of mouse lung using 19495-1-AP

IHC staining of human hepatocirrhosis using 19495-1-AP

IHC staining of human hepatocirrhosis using 19495-1-AP

IF Staining of HepG2 using 19495-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice.

Epigenetic loss of AOX1 expression via EZH2 leads to metabolic deregulations and promotes bladder cancer progression.

Evidence that fetal death is associated with placental aging.

PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.

Insight of a Metabolic Prognostic Model to Identify Tumor Environment and Drug Vulnerability for Lung Adenocarcinoma.

Aldehyde oxidase 1 promotes gallbladder carcinogenesis through ROS-mediated activation of the Wnt/β-catenin pathway