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- KD/KO Validated
AP1M1 Polyclonal antibody
AP1M1 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 12112-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue, Hacat cells |
| Positive IP detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 8 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
12112-1-AP targets AP1M1 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AP1M1 fusion protein Ag2757 相同性解析による交差性が予測される生物種 |
| Full Name | adaptor-related protein complex 1, mu 1 subunit |
| Calculated molecular weight | 423 aa, 49 kDa |
| Observed molecular weight | 49 kDa |
| GenBank accession number | BC017469 |
| Gene symbol | AP1M1 |
| Gene ID (NCBI) | 8907 |
| RRID | AB_2058339 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AP1M1 antibody 12112-1-AP | Download protocol |
| IP protocol for AP1M1 antibody 12112-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Plasma membrane contributes to the formation of pre-autophagosomal structures.
| ||
Int J Mol Sci Leucine Zipper Downregulated in Cancer-1 Interacts with Clathrin Adaptors to Control Epidermal Growth Factor Receptor (EGFR) Internalization and Gefitinib Response in EGFR-Mutated Non-Small Cell Lung Cancer | ||
Traffic AP-1A controls secretory granule biogenesis and trafficking of membrane secretory granule proteins.
| ||
J Biol Chem Adaptor Protein-1 Complex Affects the Endocytic Trafficking and Function of Peptidylglycine α-Amidating Monooxygenase, a Luminal Cuproenzyme.
| ||
Biomed Pharmacother Dioscin alleviates Alzheimer's disease through regulating RAGE/NOX4 mediated oxidative stress and inflammation. | ||
