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Validation Data Gallery

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  • Western Blot (WB) analysis of Hacat cells using AP1M2 Polyclonal antibody (10618-1-AP)

    WB analysis of Hacat cells using 10618-1-AP

    WB result of AP1M2 antibody (10618-1-AP;1:500) with siRNA cell lysate.

  • Western Blot (WB) analysis of mouse colon tissue using AP1M2 Polyclonal antibody (10618-1-AP)

    WB analysis of mouse colon using 10618-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 10618-1-AP (AP1M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using AP1M2 Polyclonal antibody (10618-1-AP)

    WB analysis of BxPC-3 using 10618-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10618-1-AP (AP1M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using AP1M2 Polyclonal antibody (10618-1-AP)

    IHC staining of human pancreas cancer using 10618-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using AP1M2 Polyclonal antibody (10618-1-AP)

    IHC staining of human pancreas cancer using 10618-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using AP1M2 Polyclonal antibody (10618-1-AP)

    IHC staining of human bowen disease using 10618-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using AP1M2 Polyclonal antibody (10618-1-AP)

    IHC staining of human bowen disease using 10618-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AP1M2 Polyclonal antibody (10618-1-AP)

    IHC staining of human kidney using 10618-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10618-1-AP (AP1M2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AP1M2 Polyclonal antibody (10618-1-AP)

    IHC staining of human kidney using 10618-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10618-1-AP (AP1M2 Antibody) at dilution of 1:50 (under 40x lens).

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  • Featured Product
  • KD/KO Validated

AP1M2 Polyclonal antibody

AP1M2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10618-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

AP1 mu2, AP-1 complex subunit mu-2, AP mu chain family member mu1B, AP 1 complex subunit mu 2, Adaptor-related protein complex 1 subunit mu-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse colon tissue, Hacat cells
Positive IHC detected inhuman pancreas cancer tissue, human kidney tissue, human bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10618-1-AP targets AP1M2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP1M2 fusion protein Ag0961 相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 1, mu 2 subunit
Calculated molecular weight 48 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC005021
Gene Symbol AP1M2
Gene ID (NCBI) 10053
RRIDAB_2242895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6Q5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AP1M2 is a subunit of the adaptor complex AP-1. It belongs to the adaptor complexes medium subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.

Protocols

Product Specific Protocols
WB protocol for AP1M2 antibody 10618-1-APDownload protocol
IHC protocol for AP1M2 antibody 10618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gene

Deciphering the influence of AP1M2 in modulating hepatocellular carcinoma growth and Mobility through JNK/ErK signaling pathway control

Authors - Huan Wang
View Article