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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AP2A1 Polyclonal antibody

AP2A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29887-1-AP

Synonyms

ADTAA, Alpha adaptin A, Alpha1 adaptin, AP 2 complex subunit alpha 1, AP2 ALPHA, AP2A1, CLAPA1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AP2A1 Polyclonal antibody (29887-1-AP)

    WB analysis using 29887-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29887-1-AP (AP2A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AP2A1 Polyclonal antibody (29887-1-AP)

    IHC staining of human ovary tumor using 29887-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29887-1-AP (AP2A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AP2A1 Polyclonal antibody (29887-1-AP)

    IHC staining of human ovary tumor using 29887-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29887-1-AP (AP2A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AP2A1 Polyclonal antibody (29887-1-AP)

    IF Staining of HeLa using 29887-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AP2A1 antibody (29887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using AP2A1 Polyclonal antibody (29887-1-AP)

    IF Staining of SH-SY5Y using 29887-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using AP2A1 antibody (29887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, SH-SY5Y cells, mouse heart tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29887-1-AP targets AP2A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP2A1 fusion protein Ag31809 相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 2, alpha 1 subunit
Calculated molecular weight108KD
Observed molecular weight100-108 kDa
GenBank accession numberNM_014203
Gene symbol AP2A1
Gene ID (NCBI) 160
RRIDAB_2918351
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.

Protocols

Product Specific Protocols
WB protocol for AP2A1 antibody 29887-1-APDownload protocol
IHC protocol for AP2A1 antibody 29887-1-APDownload protocol
IF protocol for AP2A1 antibody 29887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols