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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AP2A1 Polyclonal antibody

AP2A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30030-1-AP

Synonyms

Alpha adaptin A, alpha Adaptin, ADTAA, Adaptor-related protein complex 2 subunit alpha-1, Adaptor protein complex AP-2 subunit alpha-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AP2A1 Polyclonal antibody (30030-1-AP)

    WB analysis using 30030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30030-1-AP (AP2A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AP2A1 Polyclonal antibody (30030-1-AP)

    WB analysis using 30030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30030-1-AP (AP2A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using AP2A1 Polyclonal antibody (30030-1-AP)

    IHC staining of human stomach cancer using 30030-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30030-1-AP (AP2A1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, MCF-7 cells, SKOV-3 cells, SH-SY5Y cells, mouse kidney tissue, rat kidney tissue, mouse kidney cells, rat kidney cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30030-1-AP targets AP2A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP2A1 fusion protein Ag32232 相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 2, alpha 1 subunit
Calculated molecular weight 977 aa, 108 kDa
Observed molecular weight108 kDa
GenBank accession numberBC014214
Gene symbol AP2A1
Gene ID (NCBI) 160
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.

Protocols

Product Specific Protocols
WB protocol for AP2A1 antibody 30030-1-APDownload protocol
IHC protocol for AP2A1 antibody 30030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols