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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AP2A2 Polyclonal antibody

AP2A2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15722-1-AP

Synonyms

ADTAB, Alpha adaptin C, Alpha2 adaptin, AP 2 complex subunit alpha 2, AP2A2, CLAPA2, HIP 9, HIP9, Huntingtin yeast partner J, HYPJ, KIAA0899

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using AP2A2 Polyclonal antibody (15722-1-AP)

    WB analysis of mouse brain using 15722-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15722-1-AP (AP2A2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using AP2A2 Polyclonal antibody (15722-1-AP)

    WB analysis of rat brain using 15722-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 15722-1-AP (AP2A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using AP2A2 Polyclonal antibody (15722-1-AP)

    IHC staining of human testis using 15722-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15722-1-AP (AP2A2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using AP2A2 Polyclonal antibody (15722-1-AP)

    IHC staining of human testis using 15722-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15722-1-AP (AP2A2 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15722-1-AP targets AP2A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP2A2 fusion protein Ag8442 相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 2, alpha 2 subunit
Calculated molecular weight 939 aa, 104 kDa
Observed molecular weight 104 kDa
GenBank accession numberBC006155
Gene symbol AP2A2
Gene ID (NCBI) 161
RRIDAB_2878175
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AP2A2, also named as ADTAB, CLAPA2, HIP9, HYPJ, or KIAA0899, is a 939 amino acid protein, which belongs to the adaptor complexes large subunit family. AP2A2 localizes in the membrane and is a component of the adaptor protein complex 2. AP2 adaptor complex linking clathrin to receptors in coated vesicles, clathrin-associated protein complexes are believed to interact with cytoplasmic domains of transmembrane proteins leading to their selection and concentration. AP2A2 is invovled into cellular trafficking transport.

Protocols

Product Specific Protocols
WB protocol for AP2A2 antibody 15722-1-APDownload protocol
IHC protocol for AP2A2 antibody 15722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Emerg Microbes Infect

CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis

Authors - Xin Chen
View Article