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AP3M2 Polyclonal antibody

AP3M2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11925-1-AP

Synonyms

AP 3 complex subunit mu 2, AP3M2, AP47B, CLA20, HA1 47 kDa subunit homolog 2, Mu3B adaptin, P47B

Validation Data Gallery

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  • Western Blot (WB) analysis of Hacat cells (siRNA) using AP3M2 Polyclonal antibody (11925-1-AP)

    WB analysis of Hacat cells (siRNA) using 11925-1-AP

    WB result of AP3M2 antibody (11925-1-AP; 1:500) with siRNA cell lysate.

  • Western Blot (WB) analysis of human brain tissue using AP3M2 Polyclonal antibody (11925-1-AP)

    WB analysis of human brain using 11925-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11925-1-AP (AP3M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using AP3M2 Polyclonal antibody (11925-1-AP)

    IP experiment of mouse brain using 11925-1-AP

    IP result of anti-AP3M2 (IP:11925-1-AP, 4ug; Detection:11925-1-AP 1:300) with mouse brain tissue lysate 3200 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AP3M2 Polyclonal antibody (11925-1-AP)

    IHC staining of mouse brain using 11925-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11925-1-AP (AP3M2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AP3M2 Polyclonal antibody (11925-1-AP)

    IHC staining of mouse brain using 11925-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11925-1-AP (AP3M2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inhuman brain tissue, Hacat cells (siRNA)
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11925-1-AP targets AP3M2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP3M2 fusion protein Ag2572 相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 3, mu 2 subunit
Calculated molecular weight 418 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC056257
Gene symbol AP3M2
Gene ID (NCBI) 10947
RRIDAB_10640438
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AP3M2 is a 47-kDa protein that belongs to the adaptor complexes medium subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3M2 is a subunit of the AP-3 complex which is associated with the Golgi region as well as more peripheral structures. AP-3 is implicated in the transport of lysosomal membrane proteins.

Protocols

Product Specific Protocols
WB protocol for AP3M2 antibody 11925-1-APDownload protocol
IHC protocol for AP3M2 antibody 11925-1-APDownload protocol
IP protocol for AP3M2 antibody 11925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols