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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AP3S2 Polyclonal antibody

AP3S2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15319-1-AP

Synonyms

AP 3 complex subunit sigma 2, AP 3 complex subunit sigma 3B, AP3S2, AP3S3, Sigma 3B adaptin, Sigma adaptin 3b, sigma3b, Sigma3B adaptin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AP3S2 Polyclonal antibody (15319-1-AP)

    WB analysis using 15319-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15319-1-AP (AP3S2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using AP3S2 Polyclonal antibody (15319-1-AP)

    WB analysis of human testis using 15319-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 15319-1-AP (AP3S2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using AP3S2 Polyclonal antibody (15319-1-AP)

    WB analysis of mouse ovary using 15319-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15319-1-AP (AP3S2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AP3S2 Polyclonal antibody (15319-1-AP)

    IHC staining of human colon cancer using 15319-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AP3S2 Polyclonal antibody (15319-1-AP)

    IHC staining of human colon cancer using 15319-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AP3S2 Polyclonal antibody (15319-1-AP)

    IHC staining of human ovary tumor using 15319-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AP3S2 Polyclonal antibody (15319-1-AP)

    IHC staining of human ovary tumor using 15319-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AP3S2 Polyclonal antibody (15319-1-AP)

    IHC staining of human ovary tumor using 15319-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15319-1-AP (AP3S2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AP3S2 Polyclonal antibody (15319-1-AP)

    IHC staining of human ovary tumor using 15319-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15319-1-AP (AP3S2 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

Positive WB detected inmouse liver tissue, human testis tissue, mouse ovary tissue, mouse testis tissue, rat liver tissue
Positive IHC detected inhuman colon cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15319-1-AP targets AP3S2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP3S2 fusion protein Ag7565 相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 3, sigma 2 subunit
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC002785
Gene symbol AP3S2
Gene ID (NCBI) 10239
RRIDAB_2056644
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AP3S2 is a 22-kDa protein that belongs to the adaptor complexes small subunit family. AP3S2 is a subunit of the AP-3 complex which is associated with the Golgi region as well as more peripheral structures. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-3 complex is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2).

Protocols

Product Specific Protocols
WB protocol for AP3S2 antibody 15319-1-APDownload protocol
IHC protocol for AP3S2 antibody 15319-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols