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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AP4B1 Recombinant antibody (84710-3-RR)

    WB analysis using 84710-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84710-3-RR (AP4B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using AP4B1 Recombinant antibody (84710-3-RR)

    IP experiment of HeLa using 84710-3-RR

    IP result of anti-AP4B1 (IP:84710-3-RR, 4ug; Detection:84710-3-RR 1:1500) with HeLa cells lysate 1200 ug.

  • Affinity and Kinetic Characterization of 84710-3-RR

    Affinity and Kinetic Characterization of 84710-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84710-3-RR against Human AP4B1 were performed. The affinity constant is 47.1 pM.

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AP4B1 Recombinant antibody

AP4B1 Recombinant Antibody for WB, IP, ELISA
Cat No. 84710-3-RR
Clone No.242121D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

Beta4-adaptin, Beta subunit of AP-4, AP-4 complex subunit beta-1, AP-4 adaptor complex subunit beta, Adaptor-related protein complex 4 subunit beta-1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMDA-MB-231 cells, MCF-7 cells, HeLa cells, HuH-7 cells, SH-SY5Y cells, NIH/3T3 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84710-3-RR targets AP4B1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AP4B1 fusion protein Ag9348 相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 4, beta 1 subunit
Calculated molecular weight 739 aa, 83 kDa
Observed molecular weight~75 kDa
GenBank accession numberBC014146
Gene Symbol AP4B1
Gene ID (NCBI) 10717
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y6B7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Adaptor protein (AP) complex is a component of intracellular protein transport. The AP4 complex is involved in sorting transmembrane cargo proteins into transport vesicles for trans-Golgi network export. AP4B1 is a member of the AP4 complex, which also includes AP4E1, AP4M1 and AP4S1. Loss-of-function variants in any of these four members can lead to AP-4-associated hereditary spastic paraplegia (HSP), a neurodegenerative disorder (PMID: 30543385). This antibody recognizes AP4B1.

Protocols

Product Specific Protocols
WB protocol for AP4B1 antibody 84710-3-RRDownload protocol
IP protocol for AP4B1 antibody 84710-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols