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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AP5Z1/SPG48 Monoclonal antibody, PBS Only

AP5Z1/SPG48 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G1D7

Cat no : 66533-1-PBS

Synonyms

AP-5 complex subunit zeta-1, AP5Z1, AP5Z1/SPG48, KIAA0415, SPG48, Zeta5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AP5Z1/SPG48 Monoclonal antibody (66533-1-Ig)

    WB analysis using 66533-1-Ig (same clone as 66533-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66533-1-Ig (Zeta5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66533-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AP5Z1/SPG48 Monoclonal antibody (66533-1-Ig)

    IHC staining of human liver cancer using 66533-1-Ig (same clone as 66533-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66533-1-Ig (AP5Z1/SPG48 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66533-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AP5Z1/SPG48 Monoclonal antibody (66533-1-Ig)

    IHC staining of human liver cancer using 66533-1-Ig (same clone as 66533-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66533-1-Ig (AP5Z1/SPG48 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66533-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using AP5Z1/SPG48 Monoclonal antibody (66533-1-Ig)

    IF Staining of HEK-293 using 66533-1-Ig (same clone as 66533-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using AP5Z1/SPG48 antibody (66533-1-Ig, Clone: 1G1D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66533-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66533-1-PBS targets AP5Z1/SPG48 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AP5Z1/SPG48 fusion protein Ag26961 相同性解析による交差性が予測される生物種
Full Name KIAA0415
Calculated molecular weight 807 aa, 88 kDa
Observed molecular weight 88 kDa, 160 kDa
GenBank accession numberBC037399
Gene symbol AP5Z1/SPG48
Gene ID (NCBI) 9907
RRIDAB_2881896
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AP5Z1, also known as SPG48 and KIAA0415, is a part of AP-5. Biallelic mutations in the AP5Z1 gene encoding the AP5Z1 subunit have been described in a small number of patients with hereditary spastic paraplegia (HSP). AP5Z1 physically interacts with other HSP proteins and that patient cells are sensitive to DNA damaging drugs.