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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AP5Z1/SPG48 Monoclonal antibody, PBS Only

AP5Z1/SPG48 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G1D7

Cat no : 66533-1-PBS

Synonyms

AP-5 complex subunit zeta-1, AP5Z1, AP5Z1/SPG48, KIAA0415, SPG48, Zeta5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AP5Z1/SPG48 Monoclonal antibody (66533-1-Ig)

    WB analysis using 66533-1-Ig (same clone as 66533-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66533-1-Ig (Zeta5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66533-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AP5Z1/SPG48 Monoclonal antibody (66533-1-Ig)

    IHC staining of human liver cancer using 66533-1-Ig (same clone as 66533-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66533-1-Ig (AP5Z1/SPG48 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66533-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AP5Z1/SPG48 Monoclonal antibody (66533-1-Ig)

    IHC staining of human liver cancer using 66533-1-Ig (same clone as 66533-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66533-1-Ig (AP5Z1/SPG48 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66533-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using AP5Z1/SPG48 Monoclonal antibody (66533-1-Ig)

    IF Staining of HEK-293 using 66533-1-Ig (same clone as 66533-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using AP5Z1/SPG48 antibody (66533-1-Ig, Clone: 1G1D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66533-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66533-1-PBS targets AP5Z1/SPG48 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AP5Z1/SPG48 fusion protein Ag26961 相同性解析による交差性が予測される生物種
Full Name KIAA0415
Calculated molecular weight 807 aa, 88 kDa
Observed molecular weight 88 kDa, 160 kDa
GenBank accession numberBC037399
Gene symbol KIAA0415
Gene ID (NCBI) 9907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AP5Z1, also known as SPG48 and KIAA0415, is a part of AP-5. Biallelic mutations in the AP5Z1 gene encoding the AP5Z1 subunit have been described in a small number of patients with hereditary spastic paraplegia (HSP). AP5Z1 physically interacts with other HSP proteins and that patient cells are sensitive to DNA damaging drugs.