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APAF1 Polyclonal antibody

APAF1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21710-1-AP

Synonyms

APAF 1, APAF1, CED4, KIAA0413


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Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, COLO 320 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21710-1-AP is APAF1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APAF1 fusion protein Ag16336 相同性解析による交差性が予測される生物種
Full Name apoptotic peptidase activating factor 1
Calculated molecular weight 1248 aa, 142 kDa
Observed molecular weight 130-142 kDa
GenBank accession numberBC136532
Gene symbol APAF1
Gene ID (NCBI) 317
RRIDAB_10859252
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

Protocols

Product Specific Protocols
WB protocol for APAF1 antibody 21710-1-APDownload protocol
IHC protocol for APAF1 antibody 21710-1-APDownload protocol
IF protocol for APAF1 antibody 21710-1-APDownload protocol
IP protocol for APAF1 antibody 21710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Theranostics

Pro-Inflammatory CXCR3 Impairs Mitochondrial Function in Experimental Non-Alcoholic Steatohepatitis.

Authors - Jinghua Du
humanWB

Oxid Med Cell Longev

Apoptotic Protease Activating Factor-1 Inhibitor Mitigates Myocardial Ischemia Injury via Disturbing Procaspase-9 Recruitment by Apaf-1.

Authors - Ying Wang
humanWB,IHC,IF

Mol Ther Nucleic Acids

LNC473 Regulating APAF1 IRES-Dependent Translation via Competitive Sponging miR574 and miR15b: Implications in Colorectal Cancer.

Authors - Huizhe Wu
ratWB

Cell Biol Toxicol

Inhibition of ER stress attenuates kidney injury and apoptosis induced by 3-MCPD via regulating mitochondrial fission/fusion and Ca2+ homeostasis.

Authors - Yujie Zhong
humanWB

Front Cell Dev Biol

Antagonizing CDK8 Sensitizes Colorectal Cancer to Radiation Through Potentiating the Transcription of e2f1 Target Gene apaf1.

Authors - Bin Chen
humanWB

Cells

FUT2 Facilitates Autophagy and Suppresses Apoptosis via p53 and JNK Signaling in Lung Adenocarcinoma Cells

Authors - Yuqi Zhang