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Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
29022-1-PBS targets APAF1 in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | APAF1 fusion protein Ag30606 相同性解析による交差性が予測される生物種 |
| Full Name | apoptotic peptidase activating factor 1 |
| Calculated molecular weight | 1248 aa, 142 kDa |
| Observed molecular weight | 130-140 kDa |
| GenBank accession number | BC136532 |
| Gene Symbol | APAF1 |
| Gene ID (NCBI) | 317 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14727 |
| Storage Buffer | PBS only , pH 7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.