Validation Data Gallery
Tested Applications
Positive WB detected in | Neuro-2a cells, SH-SY5Y cells, SK-N-SH cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
55089-1-AP targets APBA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | amyloid beta (A4) precursor protein-binding, family A, member 1 |
Calculated molecular weight | 93 kDa |
Observed molecular weight | 138-140 kDa |
GenBank accession number | NM_001163 |
Gene Symbol | APBA1 |
Gene ID (NCBI) | 320 |
RRID | AB_3670237 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q02410 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
APBA1, also named MINT1 and X11A, is a brain-specific multi-modular protein that interacts with the Alzheimer's disease amyloid precursor protein (APP)(PMID: 22136355). APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. APBA1 is expressed in the brain and was found to interact with Munc18.
Protocols
Product Specific Protocols | |
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WB protocol for APBA1 antibody 55089-1-AP | Download protocol |
IHC protocol for APBA1 antibody 55089-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |