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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APCDD1 Polyclonal antibody (17024-1-AP)

    WB analysis using 17024-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17024-1-AP (APCDD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using APCDD1 Polyclonal antibody (17024-1-AP)

    IHC staining of human heart using 17024-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17024-1-AP (APCDD1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using APCDD1 Polyclonal antibody (17024-1-AP)

    IHC staining of human heart using 17024-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17024-1-AP (APCDD1 Antibody) at dilution of 1:50 (under 40x lens).

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APCDD1 Polyclonal antibody

APCDD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17024-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

APCDD1, B7323, DRAPC1, FP7019, Protein APCDD1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells, Raji cells, mouse brain tissue, mouse heart tissue, mouse skin tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17024-1-AP targets APCDD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APCDD1 fusion protein Ag10303 相同性解析による交差性が予測される生物種
Full Name adenomatosis polyposis coli down-regulated 1
Calculated molecular weight 514 aa, 59 kDa
Observed molecular weight68 kDa
GenBank accession numberBC053324
Gene Symbol APCDD1
Gene ID (NCBI) 147495
RRIDAB_3085513
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8J025
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APCDD1(adenomatosis polyposis coli down-regulated 1) is a membrane-bound glycoprotein conserved during vertebrate evolution that regulates important biological processes controlled by Wnt signaling (PMID: 26170146). APCDD1 is a membrane-bound glycoprotein that is abundantly expressed in human HFs (hair follicles) and can interact in vitro with WNT3A and LRP5, two essential components of Wnt signaling (PMID: 20393562). APCDD1 may be modified by glycosylation.

Protocols

Product Specific Protocols
WB protocol for APCDD1 antibody 17024-1-APDownload protocol
IHC protocol for APCDD1 antibody 17024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols