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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APCDD1 Recombinant antibody (83246-6-RR)

    WB analysis using 83246-6-RR (same clone as 83246-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83246-6-RR (APCDD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83246-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using APCDD1 Recombinant antibody (83246-6-RR)

    WB analysis using 83246-6-RR (same clone as 83246-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83246-6-RR (APCDD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83246-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83246-6-RR

    Affinity and Kinetic Characterization of 83246-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83246-6-RR against Human APCDD1 were performed. The affinity constant is 0.634 nM.

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APCDD1 Recombinant antibody, PBS Only

APCDD1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83246-6-PBS
Clone No.240085C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Protein APCDD1, FP7019, DRAPC1, B7323, Adenomatosis polyposis coli down-regulated 1 protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83246-6-PBS targets APCDD1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen APCDD1 fusion protein Ag10303 相同性解析による交差性が予測される生物種
Full Name adenomatosis polyposis coli down-regulated 1
Calculated molecular weight 514 aa, 59 kDa
Observed molecular weight59 kDa
GenBank accession numberBC053324
Gene Symbol APCDD1
Gene ID (NCBI) 147495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8J025
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

APCDD1(adenomatosis polyposis coli down-regulated 1) is a membrane-bound glycoprotein conserved during vertebrate evolution that regulates important biological processes controlled by Wnt signaling (PMID: 26170146). APCDD1 is a membrane-bound glycoprotein that is abundantly expressed in human HFs (hair follicles) and can interact in vitro with WNT3A and LRP5, two essential components of Wnt signaling (PMID: 20393562). APCDD1 may be modified by glycosylation.