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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APCDD1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.781-50 ng/mL

Cat No : MP00199-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00199-4, Human APCDD1 Recombinant Matched Antibody Pair - PBS only. 83246-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10303. 83246-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

    Sandwich ELISA standard curve of MP00199-4

    Sandwich ELISA standard curve of MP00199-4, Human APCDD1 Recombinant Matched Antibody Pair - PBS only. 83246-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10303. 83246-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

  • The mean APCDD1 concentration was determined to be 39.03 ng/mL in SKOV-3 cell extract based on a 4.20 mg/mL extract load, 333.27 ng/mL in Raji cell extract based on a 3.00 mg/mL extract load and 570.52 ng/mL in HEK-293T cell extract based on a 2.60 mg/mL extract load.

    Sample test of MP00199-4

    The mean APCDD1 concentration was determined to be 39.03 ng/mL in SKOV-3 cell extract based on a 4.20 mg/mL extract load, 333.27 ng/mL in Raji cell extract based on a 3.00 mg/mL extract load and 570.52 ng/mL in HEK-293T cell extract based on a 2.60 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00199-4 targets APCDD1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.781-50 ng/mL (Sandwich ELISA);

Capture antibody: APCDD1 Recombinant antibody, PBS Only (Capture/Detector) 83246-1-PBS (240085A5). 100 μg. Concentration 1 mg/ml.

Detection antibody: APCDD1 Recombinant antibody, PBS Only (Capture/Detector) 83246-2-PBS (240085D8). 100 μg. Concentration 1 mg/ml.

Alternative APCDD1 matched antibody pairs: MP00199-1, MP00199-2, MP00199-3

Matched pair protein standard: Ag10303

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83246-1-PBS
Tested Reactivity human
Immunogen APCDD1 fusion protein Ag10303
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name adenomatosis polyposis coli down-regulated 1
Synonyms Protein APCDD1, FP7019, DRAPC1, B7323, Adenomatosis polyposis coli down-regulated 1 protein
GenBank accession numberBC053324
Gene symbol APCDD1
UniprotQ8J025
Gene ID (NCBI) 147495
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83246-2-PBS
Tested Reactivity human
Immunogen APCDD1 fusion protein Ag10303
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name adenomatosis polyposis coli down-regulated 1
Synonyms Protein APCDD1, FP7019, DRAPC1, B7323, Adenomatosis polyposis coli down-regulated 1 protein
GenBank accession numberBC053324
Gene symbol APCDD1
UniprotQ8J025
Gene ID (NCBI) 147495
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.