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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Serum amyloid P component Polyclonal antibody

Serum amyloid P component Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20773-1-AP

Synonyms

APCS, SAP, PTX2, amyloid P component, serum, 9.5S alpha-1-glycoprotein

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using Serum amyloid P component Polyclonal antibody (20773-1-AP)

    WB analysis of human kidney using 20773-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Serum amyloid P component Polyclonal antibody (20773-1-AP)

    IHC staining of human liver using 20773-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Serum amyloid P component Polyclonal antibody (20773-1-AP)

    IHC staining of human liver using 20773-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using Serum amyloid P component Polyclonal antibody (20773-1-AP)

    IHC staining of human spleen using 20773-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using Serum amyloid P component Polyclonal antibody (20773-1-AP)

    IHC staining of human spleen using 20773-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human normal colon using Serum amyloid P component Polyclonal antibody (20773-1-AP)

    IHC staining of human normal colon using 20773-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman kidney tissue
Positive IHC detected inhuman liver tissue, human spleen tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

20773-1-AP targets Serum amyloid P component in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Serum amyloid P component fusion protein Ag14765 相同性解析による交差性が予測される生物種
Full Name amyloid P component, serum
Calculated molecular weight 223 aa, 25 kDa
Observed molecular weight 27-29 kDa
GenBank accession numberBC007058
Gene symbol Serum amyloid P component
Gene ID (NCBI) 325
RRIDAB_10915553
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Serum amyloid P component (SAP), a member of pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Protocols

Product Specific Protocols
WB protocol for Serum amyloid P component antibody 20773-1-APDownload protocol
IHC protocol for Serum amyloid P component antibody 20773-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.

Authors - Anika Alberts
View Article
humanWB

JACC Basic Transl Sci

Bioprosthetic Valve Deterioration: Accumulation of Circulating Proteins and Macrophages in the Valve Interstitium

Authors - Tomohisa Sakaue
View Article