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APEX1 Polyclonal antibody

Name: APEX1 Polyclonal antibody
Brand: Proteintech
SKU: 10203-1-AP

APEX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10203-1-AP

Synonyms

APEX nuclease, APEX, APEN, APE-1, APE1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 10203-1-AP
WB result of APEX1 antibody (10203-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APEX1 transfected HeLa cells.
WB analysis of HeLa using 10203-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10203-1-AP (APEX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HuH-7 using 10203-1-AP
WB result of APEX1 antibody (10203-1-AP; 1:10000; room temperature for 1.5 hours) with wild-type and APEX1 knockout HuH-7 cells.
WB analysis of mouse brain using 10203-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10203-1-AP (APEX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10203-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10203-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10203-1-AP
IP result of anti-APEX1 (IP:10203-1-AP, 4ug; Detection:10203-1-AP 1:1000) with HeLa cells lysate 1600ug.
IHC staining of human stomach cancer using 10203-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10203-1-AP (APEX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 10203-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10203-1-AP (APEX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 10203-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10203-1-AP (APEX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 10203-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 10203-1-AP (APEX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 10203-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using APEX1 antibody (10203-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Cat No. 10203-1-AP

Size/Concentration

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, mouse brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human stomach cancer tissue, human thyroid cancer tissue, mouse colon tissue, rat colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 19 publications below
IHC	See 5 publications below
IF	See 3 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

10203-1-AP targets APEX1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	APEX1 fusion protein Ag0251 相同性解析による交差性が予測される生物種
Full Name	APEX nuclease (multifunctional DNA repair enzyme) 1
Calculated molecular weight	36 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC002338
Gene symbol	APEX1
Gene ID (NCBI)	328
RRID	AB_2057927
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

APEX1, also named as APE, APE1, HAP1 and REF-1, belongs to the DNA repair enzymes AP/ExoA family. It is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are in DNA repair and redox regulation of transcriptional factors. APEX nuclease is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. APEX1 is involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). When acetylated at Lys-6 and Lys-7, APEX1 stimulates the YBX1-mediated MDR1 promoter activity, leading to drug resistance. It also acts as an endoribonuclease involved in the control of single-stranded RNA metabolism. It plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, APEX1 plays a role in the rRNA quality control process during cell cycle progression. 10203-1-AP is a rabbit polyclonal antibody raised against full length APE1 of human origin.

Protocols

Product Specific Protocols
WB protocol for APEX1 antibody 10203-1-AP	Download protocol
IHC protocol for APEX1 antibody 10203-1-AP	Download protocol
IF protocol for APEX1 antibody 10203-1-AP	Download protocol
IP protocol for APEX1 antibody 10203-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Sci Adv Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair Authors - Dinh Duc Nguyen View Article
human	WB,IHC	Mol Ther miR-1293, a Candidate for miRNA-Based Cancer Therapeutics, Simultaneously Targets BRD4 and the DNA Repair Pathway. Authors - Yuki Takagawa KD Validated View Article
rat	IF	Free Radic Biol Med Systematical exploration of transcription factors in cardiomyocytes against hydrogen peroxide-induced oxidative stress by Danhong injection. Authors - Jingjing Zhang View Article
human	WB,IP	Int Immunopharmacol Inhibitors of APE1 redox and ATM synergistically sensitize osteosarcoma cells to ionizing radiation by inducing ferroptosis Authors - Hanxi Xiao KD Validated View Article
mouse	WB	Mol Cell Biol Apurinic/apyrimidinic endonuclease 1 is the essential nuclease during immunoglobulin class switch recombination. Authors - Masani Shahnaz S View Article
rat,human	IF	Sci Rep Screening and identification of critical transcription factors involved in the protection of cardiomyocytes against hydrogen peroxide-induced damage by Yixin-shu. Authors - Jingjing Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APEX1 Polyclonal antibody

APEX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 10203-1-AP

WB analysis of HeLa using 10203-1-AP

WB analysis of HuH-7 using 10203-1-AP

WB analysis of mouse brain using 10203-1-AP

WB analysis of mouse brain using 10203-1-AP

IP experiment of HeLa using 10203-1-AP

IHC staining of human stomach cancer using 10203-1-AP

IHC staining of human thyroid cancer using 10203-1-AP

IHC staining of mouse colon using 10203-1-AP

IHC staining of rat colon using 10203-1-AP

IF Staining of MCF-7 using 10203-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair

miR-1293, a Candidate for miRNA-Based Cancer Therapeutics, Simultaneously Targets BRD4 and the DNA Repair Pathway.

Systematical exploration of transcription factors in cardiomyocytes against hydrogen peroxide-induced oxidative stress by Danhong injection.

Inhibitors of APE1 redox and ATM synergistically sensitize osteosarcoma cells to ionizing radiation by inducing ferroptosis

Apurinic/apyrimidinic endonuclease 1 is the essential nuclease during immunoglobulin class switch recombination.

Screening and identification of critical transcription factors involved in the protection of cardiomyocytes against hydrogen peroxide-induced damage by Yixin-shu.