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Validation Data Gallery

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  • Western Blot (WB) analysis of HuH-7 cells using APEX1 Monoclonal antibody (67781-1-Ig)

    WB analysis of HuH-7 using 67781-1-Ig (same clone as 67781-1-PBS)

    WB result of APEX1 antibody (67781-1-Ig; 1:15000; room temperature for 1.5 hours) with wild-type and APEX1 knockout HuH-7 cells. This data was developed using the same antibody clone with 67781-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using APEX1 Monoclonal antibody (67781-1-Ig)

    WB analysis of HeLa using 67781-1-Ig (same clone as 67781-1-PBS)

    WB result of APEX1 antibody (67781-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APEX1 transfected HeLa cells. This data was developed using the same antibody clone with 67781-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using APEX1 Monoclonal antibody (67781-1-Ig)

    WB analysis using 67781-1-Ig (same clone as 67781-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67781-1-Ig (APEX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67781-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using APEX1 Monoclonal antibody (67781-1-Ig)

    WB analysis using 67781-1-Ig (same clone as 67781-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67781-1-Ig (APEX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67781-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using APEX1 Monoclonal antibody (67781-1-Ig)

    IHC staining of mouse liver using 67781-1-Ig (same clone as 67781-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67781-1-Ig (APEX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67781-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using APEX1 Monoclonal antibody (67781-1-Ig)

    IHC staining of mouse liver using 67781-1-Ig (same clone as 67781-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67781-1-Ig (APEX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67781-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

APEX1 Monoclonal antibody, PBS Only

APEX1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67781-1-PBS
Clone No.2B10B2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, ELISA

Synonyms

APEX, APEN, APE-1, APE1, APE

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67781-1-PBS targets APEX1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen APEX1 fusion protein Ag28552 相同性解析による交差性が予測される生物種
Full Name APEX nuclease (multifunctional DNA repair enzyme) 1
Calculated molecular weight 36 kDa
Observed molecular weight36 kDa
GenBank accession numberBC002338
Gene Symbol APEX1
Gene ID (NCBI) 328
RRIDAB_2918545
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27695
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

APEX1, also named as APE, APE1, HAP1 and REF-1, belongs to the DNA repair enzymes AP/ExoA family. It is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are in DNA repair and redox regulation of transcriptional factors. APEX nuclease is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. APEX1 is involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). When acetylated at Lys-6 and Lys-7, APEX1 stimulates the YBX1-mediated MDR1 promoter activity, leading to drug resistance. It also acts as an endoribonuclease involved in the control of single-stranded RNA metabolism. It plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, APEX1 plays a role in the rRNA quality control process during cell cycle progression.