• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using APH1A Polyclonal antibody (11643-1-AP)

    WB analysis using 11643-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using APH1A Polyclonal antibody (11643-1-AP)

    WB analysis using 11643-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using APH1A Polyclonal antibody (11643-1-AP)

    WB analysis of HeLa using 11643-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using APH1A Polyclonal antibody (11643-1-AP)

    WB analysis of human brain using 11643-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using human APH1A Polyclonal antibody (11643-1-AP)

    IP experiment of HeLa using 11643-1-AP

    IP result of anti-APH1A (IP:11643-1-AP, 4ug; Detection:11643-1-AP 1:4000) with HeLa cells lysate 1600 ug.

  • Immunohistochemistry (IHC) staining of human stomach tissue using APH1A Polyclonal antibody (11643-1-AP)

    IHC staining of human stomach using 11643-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11643-1-AP (APH1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using APH1A Polyclonal antibody (11643-1-AP)

    IHC staining of human stomach using 11643-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11643-1-AP (APH1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using APH1A Polyclonal antibody (11643-1-AP)

    IF Staining of HeLa using 11643-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using APH1A antibody (11643-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

APH1A Polyclonal antibody

APH1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11643-1-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CGI-78, CGI 78, Aph-1alpha, APH-1a, Aph 1alpha

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-29 cells, HeLa cells, human brain tissue, U-937 cells, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

11643-1-AP targets APH1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APH1A fusion protein Ag2238 相同性解析による交差性が予測される生物種
Full Name anterior pharynx defective 1 homolog A (C. elegans)
Calculated molecular weight 265 aa, 29 kDa
Observed molecular weight27-32 kDa
GenBank accession numberBC015568
Gene Symbol APH1A
Gene ID (NCBI) 51107
RRIDAB_2242861
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BI3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for APH1A antibody 11643-1-APDownload protocol
IHC protocol for APH1A antibody 11643-1-APDownload protocol
IF protocol for APH1A antibody 11643-1-APDownload protocol
IP protocol for APH1A antibody 11643-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Neurobiol

Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.

Authors - Yasuteru Inoue
View Article
humanWB

Front Neurosci

Impacts of Acute Hypoxia on Alzheimer's Disease-Like Pathologies in APPswe/PS1dE9 Mice and Their Wild Type Littermates.

Authors - Feng Zhang
View Article
mouseWB

Neurosci Lett

Metformin attenuates vascular pathology by increasing expression of insulin-degrading enzyme in a mixed model of cerebral amyloid angiopathy and type 2 diabetes mellitus.

Authors - Yasuteru Inoue
View Article
humanWB

J Allergy Clin Immunol

NETs activate Notch- γ secretase signaling in hidradenitis suppurativa

Authors - Christopher B Oliveira
View Article