Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 11643-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 11643-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11643-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11643-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11643-1-AP
IP result of anti-APH1A (IP:11643-1-AP, 4ug; Detection:11643-1-AP 1:4000) with HeLa cells lysate 1600 ug.
IHC staining of human stomach using 11643-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11643-1-AP (APH1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 11643-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11643-1-AP (APH1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 11643-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using APH1A antibody (11643-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

APH1A Polyclonal antibody

APH1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11643-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CGI-78, CGI 78, Aph-1alpha, APH-1a, Aph 1alpha

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HT-29 cells, HeLa cells, human brain tissue, U-937 cells, rat brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

11643-1-AP targets APH1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	APH1A fusion protein Ag2238 相同性解析による交差性が予測される生物種
Full Name	anterior pharynx defective 1 homolog A (C. elegans)
Calculated molecular weight	265 aa, 29 kDa
Observed molecular weight	27-32 kDa
GenBank accession number	BC015568
Gene Symbol	APH1A
Gene ID (NCBI)	51107
RRID	AB_2242861
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96BI3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for APH1A antibody 11643-1-AP	Download protocol
IHC protocol for APH1A antibody 11643-1-AP	Download protocol
IF protocol for APH1A antibody 11643-1-AP	Download protocol
IP protocol for APH1A antibody 11643-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Mol Neurobiol Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression. Authors - Yasuteru Inoue View Article
human	WB	Front Neurosci Impacts of Acute Hypoxia on Alzheimer's Disease-Like Pathologies in APPswe/PS1dE9 Mice and Their Wild Type Littermates. Authors - Feng Zhang View Article
mouse	WB	Neurosci Lett Metformin attenuates vascular pathology by increasing expression of insulin-degrading enzyme in a mixed model of cerebral amyloid angiopathy and type 2 diabetes mellitus. Authors - Yasuteru Inoue View Article
human	WB	J Allergy Clin Immunol NETs activate Notch- γ secretase signaling in hidradenitis suppurativa Authors - Christopher B Oliveira View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11643-1-AP

WB analysis using 11643-1-AP

WB analysis of HeLa using 11643-1-AP

WB analysis of human brain using 11643-1-AP

IP experiment of HeLa using 11643-1-AP

IHC staining of human stomach using 11643-1-AP

IHC staining of human stomach using 11643-1-AP

IF Staining of HeLa using 11643-1-AP

APH1A Polyclonal antibody

APH1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.

Impacts of Acute Hypoxia on Alzheimer's Disease-Like Pathologies in APPswe/PS1dE9 Mice and Their Wild Type Littermates.

Metformin attenuates vascular pathology by increasing expression of insulin-degrading enzyme in a mixed model of cerebral amyloid angiopathy and type 2 diabetes mellitus.

NETs activate Notch- γ secretase signaling in hidradenitis suppurativa