API5 Polyclonal antibody

Name: API5 Polyclonal antibody
Brand: Proteintech
SKU: 25689-1-AP

API5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 25689-1-AP

Synonyms

AAC 11, AAC11, Antiapoptosis clone 11 protein, API 5, API5, apoptosis inhibitor 5, FIF, Protein XAGL

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 25689-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 25689-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 25689-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 25689-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25689-1-AP (API5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human lung using 25689-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25689-1-AP (API5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 25689-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 25689-1-AP (API5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 25689-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 25689-1-AP (API5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 25689-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using API5 antibody (25689-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 25689-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293T cells, MCF-7 cells, NIH/3T3 cells, HepG2 cells, HeLa cells
Positive IHC detected in	human lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 1 publications below

Product Information

25689-1-AP targets API5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	API5 fusion protein Ag22524 相同性解析による交差性が予測される生物種
Full Name	apoptosis inhibitor 5
Calculated molecular weight	58 kDa
Observed molecular weight	50-55 kDa
GenBank accession number	BC017709
Gene symbol	API5
Gene ID (NCBI)	8539
RRID	AB_2880196
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The human gene API5 encodes the protein Apoptosis inhibitor 5. This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of chemotherapeutic drugs. Diseases associated with API5 include colon adenocarcinoma, and cervical cancer.

Protocols

Product Specific Protocols
WB protocol for API5 antibody 25689-1-AP	Download protocol
IHC protocol for API5 antibody 25689-1-AP	Download protocol
IF protocol for API5 antibody 25689-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Sci Rep microRNA regulatory circuits in a mouse model of inherited retinal degeneration. Authors - Arpad Palfi View Article
	WB	Gene Cloning, expression pattern, and potential role of apoptosis inhibitor 5 in the termination of embryonic diapause and early embryo development of Artemia sinica. Authors - Shuang Zhang View Article
human	WB	Oncol Lett Paeonol-mediated apoptosis of hepatocellular carcinoma cells by NF-κB pathway. Authors - Qiang Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

API5 Polyclonal antibody

API5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 25689-1-AP

WB analysis of HepG2 using 25689-1-AP

WB analysis of MCF-7 using 25689-1-AP

WB analysis of NIH/3T3 using 25689-1-AP

IHC staining of human lung using 25689-1-AP

IHC staining of human lung using 25689-1-AP

IF Staining of HepG2 using 25689-1-AP