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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APOA1 Polyclonal antibody

APOA1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31265-1-AP

Synonyms

apolipoprotein A I, ApoA-I, Apo-AI, ApoAI, ApoA I

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOA1 Polyclonal antibody (31265-1-AP)

    WB analysis using 31265-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31265-1-AP (APOA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using APOA1 Polyclonal antibody (31265-1-AP)

    WB analysis using 31265-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31265-1-AP (APOA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using APOA1 Polyclonal antibody (31265-1-AP)

    IF Staining of HeLa using 31265-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using APOA1 antibody (31265-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, mouse lung tissue, mouse spleen tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31265-1-AP targets APOA1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-I
Calculated molecular weight 31 kDa
Observed molecular weight26 kDa
GenBank accession numberBC005380
Gene symbol APOA1
Gene ID (NCBI) 335
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Protocols

Product Specific Protocols
WB protocol for APOA1 antibody 31265-1-APDownload protocol
IF protocol for APOA1 antibody 31265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols