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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APOA1 Polyclonal antibody

APOA1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31265-1-AP

Synonyms

apolipoprotein A I, ApoA-I, Apo-AI, ApoAI, ApoA I

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOA1 Polyclonal antibody (31265-1-AP)

    WB analysis using 31265-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31265-1-AP (APOA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using APOA1 Polyclonal antibody (31265-1-AP)

    WB analysis using 31265-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31265-1-AP (APOA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using APOA1 Polyclonal antibody (31265-1-AP)

    IF Staining of HeLa using 31265-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using APOA1 antibody (31265-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, mouse lung tissue, mouse spleen tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31265-1-AP targets APOA1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-I
Calculated molecular weight 31 kDa
Observed molecular weight26 kDa
GenBank accession numberBC005380
Gene symbol APOA1
Gene ID (NCBI) 335
RRIDAB_3669923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Protocols

Product Specific Protocols
WB protocol for APOA1 antibody 31265-1-APDownload protocol
IF protocol for APOA1 antibody 31265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols