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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Apolipoprotein AI Recombinant antibody, PBS Only

Apolipoprotein AI Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240269F1

Cat no : 83504-1-PBS

Synonyms

APOA1, apolipoprotein A I, Apo-AI, ApoAI, ApoA I

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Apolipoprotein AI Recombinant antibody (83504-1-RR)

    WB analysis of HepG2 using 83504-1-RR (same clone as 83504-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83504-1-RR (Apolipoprotein AI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Apolipoprotein AI Recombinant antibody (83504-1-RR)

    IHC staining of human stomach cancer using 83504-1-RR (same clone as 83504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83504-1-RR (Apolipoprotein AI antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83504-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Apolipoprotein AI Recombinant antibody (83504-1-RR)

    FC experiment of HepG2 using 83504-1-RR (same clone as 83504-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Apolipoprotein AI Recombinant antibody (83504-1-RR, Clone:240269F1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83504-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83504-1-RR

    Affinity and Kinetic Characterization of 83504-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83504-1-RR against Human Apolipoprotein AI were performed. The affinity constant is 4.05 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83504-1-PBS is Apolipoprotein AI Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-I
Calculated molecular weight 31 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC005380
Gene symbol APOA1
Gene ID (NCBI) 335
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis.