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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Apolipoprotein A II Polyclonal antibody

Apolipoprotein A II Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16845-1-AP

Synonyms

APOA2, Apolipoprotein A2, ApoA-II, Apo-AII, ApoA II

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Apolipoprotein A II Polyclonal antibody (16845-1-AP)

    WB analysis of human plasma using 16845-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using Apolipoprotein A II Polyclonal antibody (16845-1-AP)

    WB analysis of human plasma using 16845-1-AP

    human serum were subjected to SDS PAGE followed by western blot with 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human plasma using Apolipoprotein A II Polyclonal antibody (16845-1-AP)

    IP experiment of human plasma using 16845-1-AP

    IP result of anti-Apolipoprotein A II (IP:16845-1-AP, 4ug; Detection:16845-1-AP 1:500) with human plasma lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using Apolipoprotein A II Polyclonal antibody (16845-1-AP)

    IHC staining of human liver using 16845-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Apolipoprotein A II Polyclonal antibody (16845-1-AP)

    IHC staining of human liver using 16845-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Apolipoprotein A II Polyclonal antibody (16845-1-AP)

    IHC staining of human colon using 16845-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Apolipoprotein A II Polyclonal antibody (16845-1-AP)

    IHC staining of human colon using 16845-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Apolipoprotein A II Polyclonal antibody (16845-1-AP)

    IHC staining of human hepatocirrhosis using 16845-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16845-1-AP (Apolipoprotein A II antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Apolipoprotein A II Polyclonal antibody (16845-1-AP)

    IHC staining of human hepatocirrhosis using 16845-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16845-1-AP (Apolipoprotein A II antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Apolipoprotein A II Polyclonal antibody (16845-1-AP)

    IF Staining of HepG2 using 16845-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein A II antibody (16845-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inhuman plasma tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue, human colon tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

16845-1-AP targets Apolipoprotein A II in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Apolipoprotein A II fusion protein Ag9863 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-II
Calculated molecular weight 100 aa, 11 kDa
Observed molecular weight 7-9 kDa (monomer), 15 kDa (dimer)
GenBank accession numberBC005282
Gene symbol APOA2
Gene ID (NCBI) 336
RRIDAB_2878324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Apolipoprotein A-II (APOA2) is a major component of high density lipoprotein (HDL) and it plays an important role in directing the fate of lipid metabolism among HDL. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer. (PMID: 29081441, 29481802)

Protocols

Product Specific Protocols
WB protocol for Apolipoprotein A II antibody 16845-1-APDownload protocol
IHC protocol for Apolipoprotein A II antibody 16845-1-APDownload protocol
IF protocol for Apolipoprotein A II antibody 16845-1-APDownload protocol
IP protocol for Apolipoprotein A II antibody 16845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.

Authors - Yanying Wang
View Article
humanWB

Exp Cell Res

Extracellular vesicle-mediated transfer of the lncRNA-TC0101441 promotes endometriosis migration/invasion.

Authors - Jun-Jun Qiu
View Article
human,mouseWB

Int J Mol Sci

Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes

Authors - Zhonghao Li
View Article