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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APOA4 Polyclonal antibody, PBS Only

APOA4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17996-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using APOA4 Polyclonal antibody (17996-1-AP)

    WB analysis of human plasma using 17996-1-AP (same clone as 17996-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 17996-1-AP (APOA4 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17996-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using APOA4 Polyclonal antibody (17996-1-AP)

    WB analysis of HepG2 using 17996-1-AP (same clone as 17996-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17996-1-AP (APOA4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17996-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using APOA4 Polyclonal antibody (17996-1-AP)

    IHC staining of human liver using 17996-1-AP (same clone as 17996-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17996-1-AP (APOA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17996-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using APOA4 Polyclonal antibody (17996-1-AP)

    IHC staining of human liver using 17996-1-AP (same clone as 17996-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17996-1-AP (APOA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17996-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17996-1-PBS targets APOA4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOA4 fusion protein Ag12454 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-IV
Calculated molecular weight 396 aa, 45 kDa
Observed molecular weight 45-46 kDa
GenBank accession numberBC074764
Gene symbol APOA4
Gene ID (NCBI) 337
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote ins secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.