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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOBEC3A Polyclonal antibody (25084-1-AP)

    WB analysis using 25084-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25084-1-AP (APOBEC3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using APOBEC3A Polyclonal antibody (25084-1-AP)

    IF Staining of THP-1 using 25084-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using APOBEC3A antibody (25084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

APOBEC3A Polyclonal antibody

APOBEC3A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25084-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Phorbolin-1, PHO1, EC:3.5.4.38, DNA dC->dU-editing enzyme APOBEC-3A, ARP3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHaCaT cells, HeLa cells
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25084-1-AP targets APOBEC3A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOBEC3A fusion protein Ag18845 相同性解析による交差性が予測される生物種
Full Name apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
Calculated molecular weight 199 aa, 23 kDa
Observed molecular weight23 kDa
GenBank accession numberBC126416
Gene Symbol APOBEC3A
Gene ID (NCBI) 200315
RRIDAB_3085769
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31941
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APOBEC3A, also known as A3A, belongs to the cytidine and deoxycytidylate deaminase family. APOBEC3A plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. APOBEC3A is expressed in several cell types, including keratinocytes and myeloid cells (PMID: 28825669). APOBEC3A has 2 isoforms with the molecular mass of 22 and 23 kDa.

Protocols

Product Specific Protocols
WB protocol for APOBEC3A antibody 25084-1-APDownload protocol
IF protocol for APOBEC3A antibody 25084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols