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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APOBEC3A Recombinant antibody

APOBEC3A Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241529G4

Cat no : 84239-4-RR

Synonyms

PHO1, EC:3.5.4.38, DNA dC->dU-editing enzyme APOBEC-3A, ARP3, A3A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOBEC3A Recombinant antibody (84239-4-RR)

    WB analysis using 84239-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84239-4-RR (APOBEC3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using APOBEC3A Recombinant antibody (84239-4-RR)

    FC experiment of A431 using 84239-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Apobec3a Recombinant Antibody (84239-4-RR, Clone:241529G4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84239-4-RR

    Affinity and Kinetic Characterization of 84239-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84239-4-RR against Human APOBEC3A were performed. The affinity constant is 0.328 nM.

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Tested Applications

Positive WB detected inrat colon tissue
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84239-4-RR is APOBEC3A Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen APOBEC3A fusion protein Ag18845 相同性解析による交差性が予測される生物種
Full Name apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
Calculated molecular weight 199 aa, 23 kDa
Observed molecular weight28 kDa
GenBank accession numberBC126416
Gene symbol APOBEC3A
Gene ID (NCBI) 200315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APOBEC3A, also known as A3A, belongs to the cytidine and deoxycytidylate deaminase family. APOBEC3A plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. APOBEC3A is expressed in several cell types, including keratinocytes and myeloid cells (PMID: 28825669). APOBEC3A has 2 isoforms with the molecular mass of 22 and 23 kDa.

Protocols

Product Specific Protocols
WB protocol for APOBEC3A antibody 84239-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols