Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 4 publications below |
| IP | See 1 publications below |
Product Information
14559-1-AP targets APOBEC3B in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | APOBEC3B fusion protein Ag6115 相同性解析による交差性が予測される生物種 |
| Full Name | apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B |
| Calculated molecular weight | 46 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC053859 |
| Gene Symbol | APOBEC3B |
| Gene ID (NCBI) | 9582 |
| RRID | AB_2878066 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UH17 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The DNA cytosine deaminase APOBEC3B was identified recently as a source of DNA damage and mutagenesis in breast, head/neck, cervix, bladder, lung, ovary, and to lesser extents additional cancer types. This enzyme is also an effector protein in the innate immune response to virus infection. APOBEC3B has 3 isoforms produced by alternative splicing with the MW of 46, 29, 43 kDa. It can also exist as a dimer and can interact with APOBEC3G.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for APOBEC3B antibody 14559-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Med Genet Multiregion whole-genome sequencing depicts intratumour heterogeneity and punctuated evolution in ovarian clear cell carcinoma. | ||
Front Oncol Correlation Between APOBEC3B Expression and Clinical Characterization in Lower-Grade Gliomas. | ||
Mol Carcinog HPV-16/18 E6-induced APOBEC3B expression associates with proliferation of cervical cancer cells and hypomethylation of Cyclin D1. | ||
Zhongguo Fei Ai Za Zhi [SWI/SNF Complex Gene Mutations Promote the Liver Metastasis of Non-small Cell Lung Cancer Cells in NSI Mice] |