☆ 3/31(月)まで!二次抗体 & FlexAbleが30%オフ! >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

APOBEC3G Monoclonal antibody

APOBEC3G Monoclonal Antibody for WB, IF, IHC, ELISA
Cat No. 60100-1-Ig
Clone No.5G10A8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

APOBEC related protein, APOBEC related protein 9, APOBEC3G, ARCD, ARP 9, ARP9, bK150C2.7, CEM 15, CEM15, MDS019

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA375 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60100-1-Ig targets APOBEC3G in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen APOBEC3G fusion protein Ag7770 相同性解析による交差性が予測される生物種
Full Name apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC024268
Gene Symbol APOBEC3G
Gene ID (NCBI) 60489
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HC16
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APOBEC3G((apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G,also known as CEM15), a member of the APOBEC family of cytidine deaminase-editing enzymes, is a potent DNA mutator that can be packaged into budding retroviruses.HA-tagged APOBEC3G can be expressed in 293T cells, which do not express APOBEC3G endogenously,in addition 22- and 24-kDa proteolytic degradation fragments can be detected by western blot(PMID:14672928). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for APOBEC3G antibody 60100-1-IgDownload protocol
IHC protocol for APOBEC3G antibody 60100-1-IgDownload protocol
IF protocol for APOBEC3G antibody 60100-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

Hepatitis B virus X protein is capable of down-regulating protein level of host antiviral protein APOBEC3G.

Authors - Ruidong Chen
humanIHC

Int J Clin Exp Med

APOBEC3G expression is correlated with poor prognosis in colon carcinoma patients with hepatic metastasis.

Authors - Huanrong Lan
Loading...