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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APOC3 Polyclonal antibody

APOC3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22262-1-AP

Synonyms

Apo CIII, ApoC III, APOC3, APOCIII, apolipoprotein C III, Apolipoprotein C3

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using APOC3 Polyclonal antibody (22262-1-AP)

    WB analysis of human plasma using 22262-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 22262-1-AP (APOC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using APOC3 Polyclonal antibody (22262-1-AP)

    IHC staining of human liver using 22262-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22262-1-AP (APOC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using APOC3 Polyclonal antibody (22262-1-AP)

    IHC staining of human liver using 22262-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22262-1-AP (APOC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman plasma
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

22262-1-AP targets APOC3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name apolipoprotein C-III
Calculated molecular weight 11 kDa
Observed molecular weight11 kDa
GenBank accession numberNM_000040
Gene symbol APOC3
Gene ID (NCBI) 345
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for APOC3 antibody 22262-1-APDownload protocol
IHC protocol for APOC3 antibody 22262-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Curr Urol

Construction and evaluation of a novel prognostic risk model of aging-related genes in bladder cancer

Authors - Delin Wang
View Article