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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APOD Monoclonal antibody

APOD Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1C6D10

Cat no : 66215-1-Ig

Synonyms

Apo D, APOD, apolipoprotein D

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using APOD Monoclonal antibody (66215-1-Ig)

    WB analysis of human plasma using 66215-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66215-1-Ig (APOD Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using APOD Monoclonal antibody (66215-1-Ig)

    WB analysis using 66215-1-Ig

    human urine sample were subjected to SDS PAGE followed by western blot with 66215-1-Ig (APOD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using APOD Monoclonal antibody (66215-1-Ig)

    WB analysis of human placenta using 66215-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66215-1-Ig (APOD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APOD Monoclonal antibody (66215-1-Ig)

    IF Staining of HepG2 using 66215-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using APOD antibody (66215-1-Ig, Clone: 1C6D10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman plasma tissue, human plasma, human urine sample, human placenta tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66215-1-Ig targets APOD in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen APOD fusion protein Ag21422 相同性解析による交差性が予測される生物種
Full Name apolipoprotein D
Calculated molecular weight 33 kDa
Observed molecular weight 30-33 kDa
GenBank accession numberBC007402
Gene symbol APOD
Gene ID (NCBI) 347
RRIDAB_2881606
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Apolipoprotein D (ApoD) is a member of the lipocalin superfamily of ligand transporters, and has been implicated in the transport of small hydrophobic molecules. ApoD is also a component of plasma high-density lipoproteins (HDL). Alteration of ApoD expression has been linked to multiple neurological disorders, including Alzheimer's disease.

Protocols

Product Specific Protocols
WB protocol for APOD antibody 66215-1-IgDownload protocol
IF protocol for APOD antibody 66215-1-IgDownload protocol
FC protocol for APOD antibody 66215-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF,FC

Immunology

Natural killer cells promote intra-cellular-infected trophoblasts survival via APOD-LRP1 axis

Authors - Xi Fang
View Article