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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using APOE Polyclonal antibody (30535-1-AP)

    WB analysis of HepG2 using 30535-1-AP

    WB result of APOE antibody (30535-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APOE transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using APOE Polyclonal antibody (30535-1-AP)

    WB analysis using 30535-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30535-1-AP (APOE antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using APOE Polyclonal antibody (30535-1-AP)

    IHC staining of human skin cancer using 30535-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 30535-1-AP (APOE antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using APOE Polyclonal antibody (30535-1-AP)

    IHC staining of human skin cancer using 30535-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 30535-1-AP (APOE antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using APOE Polyclonal antibody (30535-1-AP)

    IF Staining of mouse brain using 30535-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using APOE antibody (30535-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APOE Polyclonal antibody (30535-1-AP)

    IF Staining of HepG2 using 30535-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOE antibody (30535-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

APOE Polyclonal antibody

APOE Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 30535-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HuH-7 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30535-1-AP targets APOE in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name Apolipoprotein E
Calculated molecular weight 36 kDa
Observed molecular weight35 kDa
GenBank accession numberBC003557
Gene Symbol APOE
Gene ID (NCBI) 348
RRIDAB_3086354
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02649
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.

Protocols

Product Specific Protocols
WB protocol for APOE antibody 30535-1-APDownload protocol
IHC protocol for APOE antibody 30535-1-APDownload protocol
IF protocol for APOE antibody 30535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols