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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using APOE Monoclonal antibody (66830-1-Ig)

    WB analysis of human plasma using 66830-1-Ig (same clone as 66830-1-PBS)

    human plasma was subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at a range of dilutions from 1:5000 to 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using APOE Monoclonal antibody (66830-1-Ig)

    WB analysis using 66830-1-Ig (same clone as 66830-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using APOE Monoclonal antibody (66830-1-Ig)

    WB analysis of HepG2 using 66830-1-Ig (same clone as 66830-1-PBS)

    WB result of APOE antibody (66830-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APOE transfected HepG2 cells. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using APOE Monoclonal antibody (66830-1-Ig)

    WB analysis using 66830-1-Ig (same clone as 66830-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:3400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human blood using APOE Monoclonal antibody (66830-1-Ig)

    WB analysis of human blood using 66830-1-Ig (same clone as 66830-1-PBS)

    human blood was subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using APOE Monoclonal antibody (66830-1-Ig)

    IHC staining of mouse brain using 66830-1-Ig (same clone as 66830-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66830-1-Ig (APOE antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using APOE Monoclonal antibody (66830-1-Ig)

    IF Staining of mouse brain using 66830-1-Ig (same clone as 66830-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using APOE antibody (66830-1-Ig, Clone: 1B2C9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using APOE Monoclonal antibody (66830-1-Ig)

    IF Staining of mouse brain using 66830-1-Ig (same clone as 66830-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using APOE antibody (66830-1-Ig, Clone: 1B2C9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APOE Monoclonal antibody (66830-1-Ig)

    IF Staining of HepG2 using 66830-1-Ig (same clone as 66830-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOE antibody (66830-1-Ig, Clone: 1B2C9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using APOE Monoclonal antibody (66830-1-Ig)

    FC experiment of HepG2 using 66830-1-Ig (same clone as 66830-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug APOE Monoclonal antibody (66830-1-Ig, Clone:1B2C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

APOE Monoclonal antibody, PBS Only

APOE Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 66830-1-PBS
Clone No.1B2C9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Synonyms

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66830-1-PBS targets APOE in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen APOE fusion protein Ag28186 相同性解析による交差性が予測される生物種
Full Name Apolipoprotein E
Calculated molecular weight 36 kDa
Observed molecular weight 34-36 kDa
GenBank accession numberBC003557
Gene Symbol APOE
Gene ID (NCBI) 348
RRIDAB_2882173
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02649
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.