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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOE Recombinant antibody (84775-6-RR)

    WB analysis using 84775-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84775-6-RR (APOE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using APOE Recombinant antibody (84775-6-RR)

    FC experiment of HepG2 using 84775-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug APOE Recombinant antibody (84775-6-RR, Clone:242310B3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84775-6-RR

    Affinity and Kinetic Characterization of 84775-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84775-6-RR against Human APOE were performed. The affinity constant is below 1 pM.

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APOE Recombinant antibody

APOE Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84775-6-RR
Clone No.242310B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HuH-7 cells, human placenta
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84775-6-RR targets APOE in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name Apolipoprotein E
Calculated molecular weight 36 kDa
Observed molecular weight 34-38 kDa
GenBank accession numberBC003557
Gene Symbol APOE
Gene ID (NCBI) 348
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP02649
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight (PMID: 9831633).

Protocols

Product Specific Protocols
WB protocol for APOE antibody 84775-6-RRDownload protocol
FC protocol for APOE antibody 84775-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols